||Recombinant human NEK2 was expressed bybaculovirus in Sf9 insect cellsusing an N-terminal His tag. MW=53kDa.
||NEK 2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the NEK2 protein is almost undetectable during G1 but accumulated progressively throughout S, reaching maximal levels in late G2. NEK2 is shown to be expressed most abundantly in the testis of the adult tissues examined being localized to the nucleus.
||Baculovirus infected Sf9 cells.
||>70 % by SDS-Page.
||100 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.5 mM ATP, 0.3 mg/ml MBPsubstrate, and 0.2 ug/ml NEK2.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 40% glycerol, and 1 mM DTT.
||>6 months at –80°C.