Cat. No.: |
PTP4A1-504H |
Product Overview: |
Recombinant human PTP4A1 protein(2-173) with N-terminal His tag was expressed in anE.coliexpression system, MW= 19.8 kDa. |
Description: |
Protein tyrosine phosphatase type IVA 1 is an enzyme that in humans is encoded by the PTP4A1 gene. The protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs), which contains a PTP domain and a characteristic C-terminal prenylation motif. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. This tyrosine phosphatase is a nuclear protein, but may primarily associate with plasma membrane. The surface membrane association of this protein depends on its C-terminal prenylation. |
Source: |
E.coli. |
Purity: |
> 90%. |
Formulation: |
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT. |
Specific Activity: |
0.168 pmol/min/μg, Assay conditions: enzyme was incubated in50mM Tris pH7.4, 150mM NaCl 5mM DTT, and 12.5mM PNPP as a substrate for 2h at 37°C. Absorbance was read by Tecan spectraphotometer at 405 nm wavelength. |
Application: |
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Storage: |
>6 months at –80°C. |