||Recombinant human PTP4A1 protein(2-173) with N-terminal His tag was expressed in anE.coliexpression system, MW= 19.8 kDa.
||Protein tyrosine phosphatase type IVA 1 is an enzyme that in humans is encoded by the PTP4A1 gene. The protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs), which contains a PTP domain and a characteristic C-terminal prenylation motif. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. This tyrosine phosphatase is a nuclear protein, but may primarily associate with plasma membrane. The surface membrane association of this protein depends on its C-terminal prenylation.
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.
||0.168 pmol/min/μg, Assay conditions: enzyme was incubated in50mM Tris pH7.4, 150mM NaCl 5mM DTT, and 12.5mM PNPP as a substrate for 2h at 37°C. Absorbance was read by Tecan spectraphotometer at 405 nm wavelength.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||>6 months at –80°C.