Recombinant Human PTPN12, GST-tagged

• Cat.No.: PTPN12-1858H
• Product Overview: Recombinant human PTPN12 protein(2-300) with N-terminal GST tag was expressed in anE.coliexpression system, MW=61.4 kDa.

Specification

• Cat. No.: PTPN12-1858H
• Description: Protein tyrosine phosphatase-PEST (PTPN12), a ubiquitously expressed cytoplasmic tyrosine phosphatase, is thought to play an important role in cell adhesion and motility, cell migration, and signal transduction for antigen receptors in B and T lymphocytes. Signal transduction via tyrosine phosphorylation, normally fine-tuned by the concerted action of both protein tyrosine kinases and protein tyrosine phosphatases (PTPs), is a key mechanism in tumorigenesis. Studies suggest potential role for PTP-PEST in regulation of p130(cas) in mitogen- and cell adhesion-induced signaling events.
• Source: E.coli.
• Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
• Purity: > 90% by SDS-PAGE.
• Specific Activity: 1 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30ºC. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP.
• Application: Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
• Storage: >6 months at –80°C.

Gene Information

• Gene Name: PTPN12 protein tyrosine phosphatase, non-receptor type 12 [ Homo sapiens ]
• Synonyms: protein tyrosine phosphatase, non-receptor type 12; PTPG1; PTP-PEST; PTPN12; protein-tyrosine phosphatase G1; tyrosine-protein phosphatase non-receptor type 12; EC 3.1.3.48; OTTHUMP00000025119; Protein-tyrosine phosphatase G1
• Gene ID: 5782
• mRNA Refseq: NM_001131008
• Protein Refseq: NP_001124480
• MIM: 600079
• UniProt ID: Q05209
• Chromosome Location: 7q11.23
• Function: SH3 domain binding; hydrolase activity; protein binding; non-membrane spanning protein tyrosine phosphatase activity