||Native human APOBwas expressed in human plasma.Itsmolecular weight is 550000 Dalton.
||APOB is thedominant protein constituent of LDL. The concentration of Apo B in normalplasma is 90 mg per 100 ml. APOB is thought to stabilize lipid emulsions,serve as a cofactor and modulator of enzymatic reactions, manage export oflipids out of cells and direct lipids to target organs. APOB levels arepositively correlated with the risk of coronary disease. Apo B levels may bea more sensitive predictor of cardiovascular risk than LDL levels and do notinvolve fasting for accurate measurement. Two forms of Apo B exist: Apo B-100and Apo B-48. The first is found in VLDL and LDL and is produced by theliver. The second is found in chylomicrons and originates in the intestine.Prepared from fresh, non-frozen plasma shown to be non reactive for HbsAG,anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approvedtests. Add deionized water to original volume, aliquot and freeze unusedportion.
||Lyophilized powderin 0.01 M Sodium Deoxycholate, 0.05 M Sodium Carbonate, 0.05 M.
||> 95 percent pureby SDS-PAGE
||When stored at -80°Cproduct is stable for 3 years from date of delivery. Avoid repeatedfreeze-thaw cycles.