||Recombinant human TCTP protein produced inE.coliwas recombined with His tag at the N Terminus. It was purified by using conventional chromatography techniques, 23.9 kDa (210aa).
||TCTP, also known as Translationally controlled tumor protein, is regulated at the translational level. This protein is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients.
||> 90% by SDS - PAGE.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
||1 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMMI IYRDLISHDE MFSDIYKIRE IADGLCLEVE GKMVSRTEGN IDDSLIGGNASAEGPEGEGT ESTVITGVDI VMNHHLQETS FTKEAYKKYI KDYMKSIKGK LEEQRPERVK PFMTGAAEQI KHILANFKNY QFFIGENMNPDGMVALLDYR EDGVTPYMIF FKDGLEMEKC.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.