Recombinant Human B-cell CLL/lymphoma 2, His-tagged


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Recombinant Human B-cell CLL/lymphoma 2, His-tagged

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Cat. No. : BCL2-5361H
Product Overview : Recombinant human BCL2was expressed in E. coli.
Description : Recombinant humanBCL2 with His Tag, excluding the BH3 domain, which corresponds to amino acids93-107. This is a single, non-glycosylated polypeptide chain containing 203amino acid residues consisting of amino acids 1-92 and 108-218.
Source : E. coli
Species : Human
Form : Lyophilized from asterile filtered solution containing 10 mM Tris-HCI, pH 8.0+1 mM EDTA+250 mMNaCI.
Purity : >95% asdetermined by RP-HPLC and SDS-PAGE analyses
Endotoxin Level : <0.1 ng/ug ofBCL2
Reconstitution : Centrifuge vialprior to opening. Suspend BCL2 in 100 ul of 0.5 M Acetic acid, over night at2-4°C. Dilute 10 fold into selected buffer system. Because BCL2 has atendency to form intramolecular disulfide bond, 5 mM DTT is recommended inassay buffer. When running SDS-PAGE gel, 10 mM DTT is recommended.
Applications : Western Blot; BindingAssay
Storage : Store lyophilizedprotein at -20°C to -80°C. Reconstituted protein is stable for 1 week at 2-4°C.For long term storage, aliquot and store at -20°C to -80°C with a carrierprotein (0.1%HSA or BSA) as a stabilizer. Please note that the addition ofany carrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. Avoid repeated freeze-thawcycles.
OfficialSymbol : BCL2
Gene Name : BCL2 B-cell CLL/lymphoma 2 [ Homosapiens ]
Synonyms : BCL2; B-cellCLL/lymphoma 2; Bcl-2; PPP1R50; apoptosis regulator Bcl-2; proteinphosphatase 1, regulatory subunit 50
Gene ID : 596
mRNA Refseq : NM_000633
Protein Refseq : NP_000624
MIM : 151430
UniProt ID : P10415
Chromosome Location : 18q21.3
Pathway : ATF-2 transcriptionfactor network; Activation of BH3-only proteins; Apoptosis; B Cell ReceptorSignaling Pathway; C-MYB transcription factor network; Cholinergic synapse; Colorectalcancer; Direct p53 effectors; EPO signaling pathway; Focal Adhesion; IL-2Signaling Pathway; Immune System; Prostate cancer; Small cell lung cancer; Toxoplasmosis
Function : BH3 domain binding; channelactivity; identical protein binding; protease binding; protein binding; proteinheterodimerization activity; protein homodimerization activity; proteinphosphatase 2A binding; sequence-specific DNA binding; transcription factorbinding; ubiquitin protein ligase binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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