"BCL2" Related Products

Recombinant Human B-cell CLL/lymphoma 2, His-tagged

Cat. No. : BCL2-5361H
Product Overview : Recombinant human BCL2was expressed in E. coli.
Description : Recombinant humanBCL2 with His Tag, excluding the BH3 domain, which corresponds to amino acids93-107. This is a single, non-glycosylated polypeptide chain containing 203amino acid residues consisting of amino acids 1-92 and 108-218.
Source : E. coli
Species : Human
Form : Lyophilized from asterile filtered solution containing 10 mM Tris-HCI, pH 8.0+1 mM EDTA+250 mMNaCI.
Purity : >95% asdetermined by RP-HPLC and SDS-PAGE analyses
Endotoxin Level : <0.1 ng/ug ofBCL2
Reconstitution : Centrifuge vialprior to opening. Suspend BCL2 in 100 ul of 0.5 M Acetic acid, over night at2-4°C. Dilute 10 fold into selected buffer system. Because BCL2 has atendency to form intramolecular disulfide bond, 5 mM DTT is recommended inassay buffer. When running SDS-PAGE gel, 10 mM DTT is recommended.
Applications : Western Blot; BindingAssay
Storage : Store lyophilizedprotein at -20°C to -80°C. Reconstituted protein is stable for 1 week at 2-4°C.For long term storage, aliquot and store at -20°C to -80°C with a carrierprotein (0.1%HSA or BSA) as a stabilizer. Please note that the addition ofany carrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. Avoid repeated freeze-thawcycles.
OfficialSymbol : BCL2
Gene Name : BCL2 B-cell CLL/lymphoma 2 [ Homosapiens ]
Synonyms : BCL2; B-cellCLL/lymphoma 2; Bcl-2; PPP1R50; apoptosis regulator Bcl-2; proteinphosphatase 1, regulatory subunit 50
Gene ID : 596
mRNA Refseq : NM_000633
Protein Refseq : NP_000624
MIM : 151430
UniProt ID : P10415
Chromosome Location : 18q21.3
Pathway : ATF-2 transcriptionfactor network; Activation of BH3-only proteins; Apoptosis; B Cell ReceptorSignaling Pathway; C-MYB transcription factor network; Cholinergic synapse; Colorectalcancer; Direct p53 effectors; EPO signaling pathway; Focal Adhesion; IL-2Signaling Pathway; Immune System; Prostate cancer; Small cell lung cancer; Toxoplasmosis
Function : BH3 domain binding; channelactivity; identical protein binding; protease binding; protein binding; proteinheterodimerization activity; protein homodimerization activity; proteinphosphatase 2A binding; sequence-specific DNA binding; transcription factorbinding; ubiquitin protein ligase binding

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