"BCL2" Related Products

Recombinant Human B-cell CLL/lymphoma 2, His-tagged

Cat. No.: BCL2-5361H
Product Overview: Recombinant human BCL2was expressed in E. coli.
Description: Recombinant humanBCL2 with His Tag, excluding the BH3 domain, which corresponds to amino acids93-107. This is a single, non-glycosylated polypeptide chain containing 203amino acid residues consisting of amino acids 1-92 and 108-218.
Source: E. coli
Species: Human
Form: Lyophilized from asterile filtered solution containing 10 mM Tris-HCI, pH 8.0+1 mM EDTA+250 mMNaCI.
Purity: >95% asdetermined by RP-HPLC and SDS-PAGE analyses
Endotoxin Level: <0.1 ng/ug ofBCL2
Reconstitution: Centrifuge vialprior to opening. Suspend BCL2 in 100 ul of 0.5 M Acetic acid, over night at2-4°C. Dilute 10 fold into selected buffer system. Because BCL2 has atendency to form intramolecular disulfide bond, 5 mM DTT is recommended inassay buffer. When running SDS-PAGE gel, 10 mM DTT is recommended.
Applications: Western Blot; BindingAssay
Storage: Store lyophilizedprotein at -20°C to -80°C. Reconstituted protein is stable for 1 week at 2-4°C.For long term storage, aliquot and store at -20°C to -80°C with a carrierprotein (0.1%HSA or BSA) as a stabilizer. Please note that the addition ofany carrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. Avoid repeated freeze-thawcycles.
OfficialSymbol: BCL2
Gene Name: BCL2 B-cell CLL/lymphoma 2 [ Homosapiens ]
Synonyms: BCL2; B-cellCLL/lymphoma 2; Bcl-2; PPP1R50; apoptosis regulator Bcl-2; proteinphosphatase 1, regulatory subunit 50
Gene ID: 596
mRNA Refseq: NM_000633
Protein Refseq: NP_000624
MIM: 151430
UniProt ID: P10415
Chromosome Location: 18q21.3
Pathway: ATF-2 transcriptionfactor network; Activation of BH3-only proteins; Apoptosis; B Cell ReceptorSignaling Pathway; C-MYB transcription factor network; Cholinergic synapse; Colorectalcancer; Direct p53 effectors; EPO signaling pathway; Focal Adhesion; IL-2Signaling Pathway; Immune System; Prostate cancer; Small cell lung cancer; Toxoplasmosis
Function: BH3 domain binding; channelactivity; identical protein binding; protease binding; protein binding; proteinheterodimerization activity; protein homodimerization activity; proteinphosphatase 2A binding; sequence-specific DNA binding; transcription factorbinding; ubiquitin protein ligase binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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