||Recombinant Human UBE2D3 was expressed inE.Coli.
||UBE2D3 enzymes are human homologs of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. UbcH5a mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, IÎºBÎ±, p105) and abnormal proteins. UBE2D3 has 88% and 89% sequence identity with UbcH5a and UbcH5b respectively.
|Amino Acid Sequence:
||MHHHHHHAM ALKRINKELSDLA RDPPAQCSAGPV GDDMF HWQATIMGP NDSPYQGGVFFLTIHFPTD YPFKPPKVAFTTRIY HPNINSNGSI CLDILRS QWSPALTISK VLLSICSLLCDPNPDDPL.
||Sterile Filtered white lyophilized powder.
||Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
||Lyophilized from a 0.2Î¼m filtered concentrated (1 mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5.
||Lyophilized UBE2D3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution UBE2D3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.