Recombinant Human ULK1 protein, FLAG-tagged
Cat.No. : | ULK1-568H |
Product Overview : | Recombinant Human ULK1(2–end) fused with FLAG tag at N-terminal was expressed in FreeStyle 293-F cells. |
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- Gene Information
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Description : | ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner. |
Source : | FreeStyle 293-F cells |
Species : | Human |
Tag : | FLAG |
Form : | Aqueous buffer solution |
Molecular Mass : | 113 kDa |
Purity : | 0.4 |
Applications : | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Stability : | >6 months at -80 centigrade. Avoid freeze/thaw cycles. |
Gene Name : | ULK1 unc-51-like kinase 1 (C. elegans) [ Homo sapiens ] |
Official Symbol : | ULK1 |
Synonyms : | ULK1; unc-51-like kinase 1 (C. elegans); unc 51 (C. elegans) like kinase 1; serine/threonine-protein kinase ULK1; ATG1; ATG1 autophagy related 1 homolog (S. cerevisiae); ATG1A; ATG1 autophagy related 1 homolog; autophagy-related protein 1 homolog; UNC51; hATG1; Unc51.1; FLJ38455; FLJ46475; |
Gene ID : | 8408 |
mRNA Refseq : | NM_003565 |
Protein Refseq : | NP_003556 |
MIM : | 603168 |
UniProt ID : | O75385 |
Chromosome Location : | 12q24.3 |
Pathway : | Regulation of autophagy, organism-specific biosystem; Regulation of autophagy, conserved biosystem; Senescence and Autophagy, organism-specific biosystem; mTOR signaling pathway, organism-specific biosystem; mTOR signaling pathway, conserved biosystem; |
Function : | ATP binding; nucleotide binding; protein binding; protein complex binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity; |
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◆ Lysates | ||
ULK1-505HCL | Recombinant Human ULK1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionULK1 interacts with several autophagy-related proteins, including ATG13, FIP200, and ATG101, forming a complex that is essential for the initiation of autophagy.
mTOR (mechanistic target of rapamycin) is a known upstream regulator of ULK1. When nutrients are abundant, mTOR is active and inhibits ULK1. Conversely, under nutrient starvation, mTOR is inactivated, leading to ULK1 activation and initiation of autophagy.
ULK1 is a serine/threonine-protein kinase that plays a crucial role in the initiation of autophagy, a cellular process responsible for the degradation and recycling of cellular components.
Dysregulation of ULK1 can lead to impaired autophagy, which has been implicated in several diseases, including cancer. Overactive or underactive ULK1 can disrupt cellular homeostasis and contribute to tumorigenesis.
ULK1 undergoes several post-translational modifications, including phosphorylation and ubiquitination, which can either activate or inhibit its kinase activity and, consequently, its role in autophagy.
ULK1 is part of a complex that is essential for the initiation of autophagy. Upon activation, ULK1 phosphorylates several targets, leading to the nucleation and formation of the autophagosome.
ULK1 activity is sensitive to nutrient availability. Under nutrient-rich conditions, ULK1 is inhibited, while under nutrient-deprived conditions, ULK1 becomes activated, promoting autophagy to recycle cellular components and generate energy.
Customer Reviews (3)
Write a reviewReliable results, will reorder.
Affordable and high-quality.
Met all my expectations.
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