Recombinant Human ULK1 protein, FLAG-tagged

• Cat.No.: ULK1-568H
• Product Overview: Recombinant Human ULK1(2–end) fused with FLAG tag at N-terminal was expressed in FreeStyle 293-F cells.

Specification

• Description: ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
• Source: FreeStyle 293-F cells
• Species: Human
• Tag: FLAG
• Form: Aqueous buffer solution
• Molecular Mass: 113 kDa
• Purity: 0.4
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >6 months at -80 centigrade. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: ULK1 unc-51-like kinase 1 (C. elegans) [ Homo sapiens ]
• Official Symbol: ULK1
• Synonyms: ULK1; unc-51-like kinase 1 (C. elegans); unc 51 (C. elegans) like kinase 1; serine/threonine-protein kinase ULK1; ATG1; ATG1 autophagy related 1 homolog (S. cerevisiae); ATG1A; ATG1 autophagy related 1 homolog; autophagy-related protein 1 homolog; UNC51; hATG1; Unc51.1; FLJ38455; FLJ46475
• Gene ID: 8408
• mRNA Refseq: NM_003565
• Protein Refseq: NP_003556
• MIM: 603168
• UniProt ID: O75385
• Chromosome Location: 12q24.3
• Pathway: Regulation of autophagy, organism-specific biosystem; Regulation of autophagy, conserved biosystem; Senescence and Autophagy, organism-specific biosystem; mTOR signaling pathway, organism-specific biosystem; mTOR signaling pathway, conserved biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein complex binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity