Recombinant Human Platelet-Derived Growth Factor Beta Polypeptide

• Cat.No.: PDGFB-661H
• Product Overview: Recombinant PDGFB isa disulfide-linked homodimeric protein consisting of two 109 amino acidresidue subunits. Recombinant PDGFB migrates due to glycosylation as anapproximately 32 kDa protein under non-reducing conditions and as 16kDa underreducing conditions in SDS-PAGE, and was lyophilized from a 0.2 μm filtered0.02M acetic acid solution pH 6.5.

Specification

• Cat. No.: PDGFB-661H
• Description: PDGFB is synthesizedmainly by megakaryocytes. It is stored in the alpha granules of plateletsfrom which it is released after cell activation of platelets. Platelets synthesizea mixture of the three possible isoforms (BB,AB,AA)while fibroblastsstimulated with EGF synthesize AA homodimers.
• Source: Human
• Host: Saccharomycescerevisiae
• Purity: >98%, asdetermined by SDS-PAGE and HPLC.
• Sequence: MNRCWALFLS LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHGDPGEEDGAEL DLNMTRSHSG GELESLARGR RSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLVWPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVTRSPGGSQEQR AKTPQTRVTI RTVRVRRPPK GKHRKFKHTH DKTALKETLG A
• Molecular Weight: Human PDGFB,generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 16 kDa.
• Biological Activity: Activity wasdetermined by the ability to induce chemotaxis of purified eosinophils atconcentrations ranging between 50-100 ng/ml.
• Endotoxin: TheED(50) was determined by the dose-dependent proliferation of Balb/c 3T3cells.The expected ED50 for this effect is 1.0-3.0 ng/ml, corresponding to1x10⁶ IU/mg.
• Reconstitution: A quick spin of thevial followed by reconstitution in distilled water to a concentration notless than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Storage: Thelyophilized protein is stable for at least 2 years from date of receipt at-20°C. Upon reconstitution, this cytokine can be stored in working aliquotsat 2°C - 8°C for one month, or at -20°C for six months, with a carrierprotein without detectable loss of activity. Avoid repeated freeze/thawcycles.
• OfficialSymbol: PDGFB
• Pathways: Cytokine-cytokinereceptor interaction; Focal Adhesion; Down-stream signal transduction; Formationof Platelet plug; Gap junction; Glioma; HTLV-I infection; Hemostasis; Melanoma;Osteoblast Signaling; Pathways in cancer; Platelet Activation; Prostatecancer; Renal cell carcinoma; Signaling by PDGF

Gene Information

• Gene Name: PDGFB platelet-derived growth factor beta polypeptide [ Homosapiens ]
• Synonyms: PDGFB; platelet-derived growth factorbeta polypeptide; SIS; SSV; PDGF2; c-sis; FLJ12858; platelet-derivedgrowth factor subunit B; PDGF-2; becaplermin; PDGF, B chain; PDGF subunit B;OTTHUMP00000198980; OTTHUMP00000198981; proto-oncogene c-Sis;platelet-derived growth factor 2; platelet-derived growth factor B chain;platelet-derived growth factor, B chain; Platelet-derived growth factor, betapolypeptide (oncogene SIS); platelet-derived growth factor beta polypeptide(simian sarcoma viral (v-sis) oncogene homolog)
• Gene ID: 5155
• mRNA Refseq: NM_002608
• Protein Refseq: NP_002599
• MIM: 190040
• UniProt ID: P01127
• Chromosome Location: 22q13
• Function: cellsurface binding; eukaryotic cell surface binding; collagen binding; growthfactor activity; platelet-derived growth factor binding; platelet-derivedgrowth factor receptor binding; contributes_to platelet-derived growth factorreceptor binding; protein binding; protein binding; proteinheterodimerization activity; protein tyrosine kinase activity; superoxide-generatingNADPH oxidase activator activity