||Recombinant human TSSC4 protein, fused to His-tag at N-terminus,was expressed in E.coli and purified by using conventional chromatographytechniques.
||TSSC4belongs to the TSSC4 family. This protein is one of severaltumor-suppressing subtransferable fragments located in the imprinted genedomain of 11p15.5, an important tumor-suppressor gene region. Alterations inthis region have been associated with the Beckwith-Wiedemann syndrome, Wilmstumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, andbreast cancer.
||Liquid. In 20 mMTris-HCl buffer (pH 8.0) containing 2 mM DTT, 20% glycerol, 200 mM NaCl.
||36.8 kDa (353 aa),confirmed by MALDI-TOF.
||> 90%by SDS-PAGE
||0.5 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMAEAGT GEPSPSVEGE HGTEYDTLPS DTVSLSDSDS DLSLPGGAEVEALSPMGLPG EEDSGPDEPP SPPSGLLPAT VQPFHLRGMS STFSQRSRDI FDCLEGAARR APSSVAHTSMSDNGGFKRPL APSGRSPVEG LGRAHRSPAS PRVPPVPDYV AHPERWTKYS LEDVTEVSEQ SNQATALAFLGSQSLAAPTD CVSSFNQDPS SCGEGRVIFT KPVRGVEARH ERKRVLGKVG EPGRGGLGNP ATDRGEGPVELAHLAGPGSP EAEEWGSPHG GLQEVEALSG SVHSGSVPGL PPVETVGFHG SRKRSRDHFR NKSSSPEDPGAEV
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.