||Recombinanthuman CAMK4 protein, fused to His-tag at N-terminus, was expressedin E.coli and purified by conventional chromatography.
||CAMK4belongsto the serine/threonine protein kinase family, and to theCa(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is amultifunctional serine/threonine protein kinase with limited tissuedistribution, that has been implicated in transcriptional regulation inlymphocytes, neurons and male germ cells. Recombinant human CAMK4 protein,fused to His-tag at N-terminus, was expressed in E.coli and purified by usingconventional chromatography.
||Liquid.20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
||> 85% by SDS-PAGE
||0.5 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMLKVTV PSCSASSCSS VTASAAPGTA SLVPDYWIDGSNRDALSDFF EVESELGRGA TSIVYRCKQK GTQKPYALKV LKKTVDKKIV RTEIGVLLRL SHPNIIKLKEIFETPTEISL VLELVTGGEL FDRIVEKGYY SERDAADAVK QILEAVAYLH ENGIVHRDLK PENLLYATPAPDAPLKIADF GLSKIVEHQV LMKTVCGTPG YCAPEILRGC AYGPEVDMWS VGIITYILLC GFEPFYDERG DQFMFRRILNCEYYFISPWW DEVSLNAKDL VRKLIVLDPK KRLTTFQALQ HPWVTGKAAN FVHMDTAQKK LQEFNARRKLKAAVKAVVAS SRLGSASSSH GSIQESHKAS RDPSPIQDGN EDMKAIPEGE KIQGDGAQAA VKGAQAELMKVQALEKVKGA DINAEEAPKM VPKAVEDGIK VADLELEEGL AEEKLKTVEE AAAPREGQGS SAVGFEVPQQDVILPEY
||Can be storedat +4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Activation of CaMKIV; Ca-dependent events; Ca-dependent events; Calcium signaling pathway;Calmodulin induced events; Calmodulin induced events; Energy Metabolism;G-protein mediated events; Long-term potentiation; Mitochondrial GeneExpression; Neurotrophin signaling pathway; Opioid Signalling; Osteoclastdifferentiation; PLC beta mediated events; Signalling by NGF