||Recombinanthuman DCTPP1 protein, fused to His-tag at N-terminus, was expressedin E.coli and purified by conventional chromatography.
||DCTPP1,also known as dCTP pyrophosphatase 1, contains an epilepsy-associated repeat(EAR) domain that is predicted to participate in protein-proteininteractions, most likely involved in ligand recognition. This proteinfunctions to hydrolyze abnormal nucleotide triphosphates (NTPs) in cancercells that, if unregulated, could be incorporated into nascent DNA or RNA.Recombinant human DCTPP1 protein, fused to His-tag at N-terminus, wasexpressed in E.coli and purified by using conventional chromatography.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
||21.2 kDa (194aa),confirmed by MALDI-TOF.
||> 90% by SDS-PAGE
||0.5 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMSVAGG EIRGDTGGED TAAPGRFSFS PEPTLEDIRR LHAEFAAERDWEQFHQPRNL LLALVGEVGE LAELFQWKTD GEPGPQGWSP RERAALQEELSDVLIYLVAL AARCRVDLPL AVLSKMDINR RRYPAHLARS SSRKYTELPH GAISEDQAVG PADIPCDSTGQTST
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.