||Recombinant humanPDCL3 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||PDCL3 is a member ofthe phosducin-like protein family and is a putative modulator ofheterotrimeric G proteins. The protein shares extensive amino acid sequencehomology with phosducin. Members of the phosducin-like protein family havebeen shown to bind to the beta-gamma subunits of G proteins.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
||30.0 kDa(262aa), confirmed by MALDI-TOF.
||> 90%by SDS - PAGE
||1.0mg/ml (determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSMQDPNAD TEWNDILRKK GILPPKESLK ELEEEAEEEQ RILQQSVVKTYEDMTLEELE DHEDEFNEED ERAIEMYRRR RLAEWKATKL KNKFGEVLEISGKDYVQEVT KAGEGLWVIL HLYKQGIPLC ALINQHLSGL ARKFPDVKFI KAISTTCIPN YPDRNLPTIFVYLEGDIKAQ FIGPLVFGGM NLTRDELEWK LSESGAIMTD LEENPKKPIE DVLLSSVRRS VLMKRDSDSEGD
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C.Avoid repeated freezing and thawing cycles.