||Recombinant humanZFAND3 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||ZFAND3,also known as AN1-type zinc finger protein 3, contains DNA-binding domain andhas a wide variety of functions, most of which encompass some form oftranscriptional activation or repression. ZFAND3 is a 251 amino acid proteincontaining two AN1-type zinc fingers and two UIM (ubiquitin-interactingmotif) repeats. Conserved in animals and plants, the AN1-type zinc fingerdomain is often found in proteins that contain a ubiquitin-like domain, whichsuggests a role in the ubiquitination pathway.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 30% glycerol 0.1M NaCl, 1mM DTT.
||27.7 kDa(251aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appearhigher).
||> 90%by SDS - PAGE
||0.5mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMGDAGS ERSKAPSLPP RCPCGFWGSS KTMNLCSKCF ADFQKKQPDDDSAPSTSNSQ SDLFSEETTS DNNNTSITTP TLSPSQQPLP TELNVTSPSKEECGPCTDTA HVSLITPTKR SCGTDSQSEN EASPVKRPRL LENTERSEET SRSKQKSRRR CFQCQTKLELVQQELGSCRC GYVFCMLHRL PEQHDCTFDH MGRGREEAIM KMVKLDRKVG RSCQRIGEGC S
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.