||Recombinant humanDUSP10 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
||DUSP10 (Dualspecificity protein phosphatase 10) belongs to the protein-tyrosinephosphatase family. Dual specificity protein phosphatases(DUSPs) inactivatetheir target kinases by dephosphorylating both the phosphoserine/threonineand phosphotyrosine residues. They negatively regulate members of the MAPKsuperfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellularproliferation and differentiation. DUSP10 has been shown to interact withMAPK14 and MAPK8. When expressed in mammalian cells, DUSP10 blocks theenzymatic activation of MAP kinases with the selectivity p38 approximatelyJNK/SAPK >> ERK.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200mM NaCl.
||40.4kDa(359aa), confirmed by MALDI-TOF.
||> 90%by SDS - PAGE
||0.25 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMIIYPN DLAKKMTKCS KSHLPSQGPV IIDCRPFMEYNKSHIQGAVH INCADKISRR RLQQGKITVLDLISCREGKD SFKRIFSKEI IVYDENTNEP SRVMPSQPLHIVLESLKREG KEPLVLKGGL SSFKQNHENL CDNSLQLQEC REVGGGASAA SSLLPQPIPT TPDIENAELTPILPFLFLGN EQDAQDLDTM QRLNIGYVIN VTTHLPLYHY EKGLFNYKRL PATDSNKQNL RQYFEEAFEFIEEAHQCGKG LLIHCQAGVS RSATIVIAYL MKHTRMTMTD AYKFVKGKRP IISPNLNFMG QLLEFEEDLNNGVTPRILTP KLMGVETVV
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.