Recombinant Human CCAAT/Enhancer Binding Protein (C/EBP), Alpha, His-tagged

• Cat.No.: CEBPA-5377H
• Product Overview: Recombinant humanCEBP gamma is a single, non-glycosylated polypeptide chain containing aminoacids 39-147 and a His tag. Itsmolecular weight is 16500 Dalton.

Specification

• Cat. No.: CEBPA-5377H
• Description: CEBPA is a proteinthat in humans is encoded by the CEBPA gene. The protein encoded by thisintronless gene is a bZIP transcription factor which can bind as a homodimerto certain promoters and enhancers. It can also form heterodimers with therelated proteins CEBP-beta and CEBP-gamma. The encoded protein has been shownto bind to the promoter and modulate the expression of the gene encodingleptin, a protein that plays an important role in body weight homeostasis.Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibitingthese kinases and causing growth arrest in cultured cells.
• Source: E. coli
• Species: Human
• Form: Sterilefiltered liquid in 20 mM Tris-HCl, pH7.5 + 0.1 M NaCl + 5 mM β-mercaptoethanol.
• Molecular Weight: 16.5 kDa
• Purity: > 95% asdetermined by RP-HPLC and SDS-PAGE analyses
• Endotoxin Level: < 0.1 ng/µg ofCEBPA gamma
• Storage: Store at 2-4°C forup to 4 weeks or in working aliquots at -20°C for longer storage. Add a carrierprotein (0.1% HSA or BSA) as a stabilizer for long term storage. Please notethat the addition of any carrier protein into this product may produceunwanted endotoxin. This depends upon the particular application employed.Avoid repeated freeze-thaw cycles.
• OfficialSymbol: CEBPA

Gene Information

• Gene Name: CEBPA CCAAT/enhancer binding protein(C/EBP), alpha [ Homo sapiens ]
• Synonyms: CEBPA;CCAAT/enhancer binding protein (C/EBP), alpha; C/EBP-alpha; CEBP;CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer binding proteinalpha
• Gene ID: 1050
• mRNA Refseq: NM_004364
• Protein Refseq: NP_004355
• MIM: 601626
• UniProt ID: P49715
• Chromosome Location: 19q13.1
• Pathway: Acute myeloidleukemia; Adipogenesis; C-MYB transcription factor network; DevelopmentalBiology; E2F transcription factor network; EGFR1 Signaling Pathway; FOXA2 andFOXA3 transcription factor networks; Pathways in cancer; Regulation ofAndrogen receptor activity; Regulation of retinoblastoma protein;Transcriptional Regulation of White Adipocyte Differentiation; Validatedtargets of C-MYC transcriptional repression
• Function: DNA binding; proteinbinding; protein complex binding; protein domain specific binding; proteinheterodimerization activity; protein homodimerization activity;sequence-specific DNA binding; sequence-specific DNA binding transcriptionfactor activity; sequence-specific distal enhancer binding RNA polymerase IItranscription factor activity; transcription factor binding; transcriptionregulatory region DNA binding