Native Pig Creatine Kinase, Muscle


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Native Pig Creatine Kinase, Muscle

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Cat. No. : CKM-368P
Product Overview : Native Pig CKM wasexpressed in porcine heart.
Description : Creatine Kinase(CKM) catalyses the conversion of Creatine and consumes AdenosineTriphosphate (ATP) to create Phosphocreatine and Adenosine Diphosphate (ADP).This CKM enzyme reaction is reversible, such that also ATP can be generatedfrom PCr and ADP. In tissues and cells that consume ATP rapidly, especiallyskeletal muscle, but also brain, photoreceptor cells of the retina, haircells of the inner ear, spermatozoa and smooth muscle, phosphocreatine servesas an energy reservoir for the rapid buffering and regeneration of ATP insitu, as well as for intracellular energy transport by the phosphocreatineshuttle or circuit. Thus creatine kinase is an important enzyme in suchtissues. Clinically, creatine kinase is assayed in blood tests as a marker ofmyocardial infarction (heart attack), rhabdomyolysis (severe musclebreakdown), muscular dystrophy, and in acute renal failure.
Source : Porcine heart
Species : Pig
Concentration : Typically > 0.5mg Protein/mg solid by Coomassie.
Appearance : Pink to mediumbrown.
Form : Lyophilized from 20mM Tris + 1mM DTT + 1mM EDTA.
Biological Activity : One Unit willtransfer one mmole of phosphate from Creatine Phosphate to ADP per Minute at37°C. Measured at 340 nm as one equimolar amount of NADH produced by acoupled reaction.
Specific Activity : Typically > 200U/mg at 37°C
Storage : Store at -20°C.Stable for 2 years from delivery. Avoid repeated freeze-thaw cycles.
OfficialSymbol : CKM
Gene Name : CKM creatine kinase, muscle [ Sus scrofa]
Synonyms : CKM; creatinekinase, muscle; CK; CPK
Gene ID : 397264
mRNA Refseq : NM_001129949
Protein Refseq : NP_001123421
UniProt ID : Q5XLD3
Pathway : Arginine and prolinemetabolism; Metabolic pathways

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