Recombinant Human Fibroblast Growth Factor 7, His-tagged

Cat. No.: FGF7-682H
Product Overview: Recombinant human FGF7contains 164 amino acids and a 16 aa His-tag. Total length is 180 aa.
Description: The protein encoded bythis gene is a member of the fibroblast growth factor (FGF) family. FGFfamily members possess broad mitogenic and cell survival activities, and areinvolved in a variety of biological processes, including embryonicdevelopment, cell growth, morphogenesis, tissue repair, tumor growth andinvasion. This protein is a potent epithelial cell-specific growth factor,whose mitogenic activity is predominantly exhibited in keratinocytes but notin fibroblasts and endothelial cells.
Source: Hordeum vulgare(barley grain)
Species: Human
Form: Lyophilized from a0.2 µm sterile filtered solution of PBS, pH 7.2.
Molecular Weight: Predicted MW 21.2kDa including his-tag. The glycosylated recombinant protein migrates with anapparent MW 30 kDa in SDS-PAGE.
Purity: > 98% by SDS-PAGE.Purified product carries no pyrogenic or pro-inflammatory contaminants, asassayed with monocyte activation test using custom human Multiplex CytokineAssay measuring IL-6, TNF-alpha and IL-1beta induction.
Endotoxin Level: < 0.005 ng perµg of product (0.05 EU/µg) as measured by turbidimetric kinetic assay.
Biological Activity: Bioactivity isassayed by measuring the dose dependent effect of human FGF7 on proliferationof mouse BaF3. The ED50 for a typical batch of recombinant human FGF7is 0.20 ng/ml.
Specific Activity: 5.0 x 106U/mg
Cross-Reactivity: Considerablespecies cross-reactivity, including with mouse, monkey, and pig cells.
Reconstitution: Centrifuge vialprior to opening. First addsterile distilled water to the vial to fully solubilize the protein to aconcentration not less than 100 µg/ml. After complete solubilization of theprotein, it can be further diluted to other aqueous solutions.
Storage: The lyophilizedprotein, though stable at room temperature for two weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.
OfficialSymbol: FGF7
Gene Name: FGF7 fibroblast growth factor 7[ Homo sapiens ]
Synonyms: FGF7; fibroblastgrowth factor 7; KGF; HBGF-7;keratinocyte growth factor; FGF-7; OTTHUMP00000162252; heparin-binding growthfactor 7
Gene ID: 2252
mRNA Refseq: NM_002009
Protein Refseq: NP_002000
MIM: 148180
UniProt ID: P21781
Chromosome Location: 15q15-q21.1
Pathway: Downstream signalingof activated FGFR; FGFR ligand binding and activation; FGFR2 ligand bindingand activation; FGFR2b ligand binding and activation; FRS2-mediated cascade; Glypican3 network; IRS-mediated signalling; IRS-related events; Insulin receptorsignalling cascade; MAPK signaling pathway; Melanoma; Negative regulation ofFGFR signaling; PI-3K cascade; Pathways in cancer; Phospholipase C-mediatedcascade; SHC-mediated cascade
Function: chemoattractant activity;growth factor activity; heparin binding; type 2 fibroblast growth factorreceptor binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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