Recombinant Human Kinase Insert Domain Receptor (a type III receptor tyrosine kinase), GST-tagged

• Cat.No.: KDR-5446H
• Product Overview: Recombinant human KDR(aa 805-1356) has a N-terminal GST Tag and has a MW=87.0 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 805-1356 a.a.
• Description: Kinase insertdomain receptor (KDR, a type III receptor tyrosine kinase) also known asvascular endothelial growth factor receptor 2 (VEGFR-2) is a VEGF receptor.KDR is the human gene encoding it. KDR has also been designated as CD309(cluster of differentiation 309). KDR is also known as Flk1 (Fetal LiverKinase 1).
• Form: Liquid containing 25mM Tris-HCL, pH 8.0 + 100 mM NaCl + 0.05% Tween-20 + 50% glycerol + 10 mMreduced glutathione + 3 mM DTT
• Molecular Weight: 87.0 kDa
• Purity: >90% asdetermined by SDS-PAGE
• Endotoxin Level: Less than 0.1 ng/µgof protein
• Biological Activity: One unit is definedas the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrateper minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2,3 mM MnCl2, 1 mM DTT, 3 µM Na-orthovanadate, 0.5 mM ATP, 30 µg/ml Poly(Glu:Tyr) 4:1 substrate, 1 µg/ml recombinant VEGFR2.
• Applications: Useful for thestudy of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: Stable for more than6 months when stored at -80°C.
• OfficialSymbol: KDR

Gene Information

• Gene Name: KDR kinase insert domainreceptor (a type III receptor tyrosine kinase) [ Homo sapiens ]
• Synonyms: KDR; kinase insertdomain receptor (a type III receptor tyrosine kinase); FLK1; CD309; VEGFR;VEGFR2; vascular endothelial growth factor receptor 2; soluble VEGFR2;OTTHUMP00000158928; fetal liver kinase 1; fetal liver kinase-1;protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factorreceptor; EC 2.7.10; EC 2.7.10.1
• Gene ID: 3791
• mRNA Refseq: NM_002253
• Protein Refseq: NP_002244
• MIM: 191306
• UniProt ID: P35968
• Chromosome Location: 4q11-q12
• Pathway: Angiogenesis;Cytokine-cytokine receptor interaction; Endocytosis; Focal Adhesion;HIF-2-alpha transcription factor network; Id Signaling Pathway; Integrins inangiogenesis; Neurophilin interactions with VEGF and VEGFR; Notch-mediatedHES/HEY network; S1P1 pathway; Signal Transduction; Signaling events mediatedby VEGFR1 and VEGFR2; VEGF binds to VEGFR leading to receptor dimerization;VEGF ligand-receptor interactions; VEGF signaling pathway
• Function: ATP binding; Hsp90protein binding; growth factor binding; integrin binding; nucleotide binding;protein binding; protein tyrosine kinase activity; receptor activity;receptor signaling protein tyrosine kinase activity; transmembrane receptorprotein tyrosine kinase activity; vascular endothelial growthfactor-activated receptor activity