||Recombinant human RHOD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques(18-207aa).
||Rho-related GTP-binding protein, also known as RHOD, belongs to the small GTPase superfamily. The small GTPase RHOD contributes to rearrangement of the actin cytoskeleton and cell surface and also governs endosome motility and distribution.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT,50% glycerol, 200mM NaCl, 2mM EDTA, 0.1mM PMSF
||23.8kDa (211aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.25mg/ml (determined by Bradford)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MVKVVLVGDG GCGKTSLLMV FADGAFPESY TPTVFERYMV NLQVKGKPVH LHIWDTAGQD DYDRLRPLFY PDASVLLLCF DVTSPNSFDN IFNRWYPEVN HFCKKVPIIV VGCKTDLRKD KSLVNKLRRN GLEPVTYHRG QEMARSVGAV AYLECSARLH DNVHAVFQEA AEVALSSRGR NFWRRITQGF C
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeatedfreezing and thawing cycles.