Recombinant Human Neuregulin 1, HQ-tagged

• Cat.No.: NRG1-1280H
• Product Overview: Recombinant humanNRG1 contains 62 amino acids and a 16 aa HQ tag. Total length is 78 aa.

Specification

• Species: Human
• Source: Hordeum Vulgare
• Tag: HQ
• Description: NRG1 is asignaling protein for ErbB2/ErbB4 receptor heterodimers on the cardiac musclecells, playing an important role in heart structure and function throughinducing ErbB2/ErbB4 receptor phosphorylation and cardiomyocytedifferentiation. Research on molecular level discovered that recombinantneuregulin could make disturbed myocardial cell structure into order andstrengthen the connection between myocardial cells by intercalated discsre-organization.
• Form: Lyophilized from a0.2 umg sterile filtered solution of PBS, pH 7.2.
• Purity: > 98%by SDS-PAGE. Purified product carries no pyrogenic or pro-inflammatorycontaminants, as assayed with monocyte activation test using custom humanMultiplex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.
• Endotoxin Level: < 0.005ng per ug of product (0.05 EU/ug) as measured by turbidimetric kinetic assay.
• Biological Activity: Determinedby dose-dependent cell proliferation assay for MCF-7 cells. ED50for a typical batch of NRG1 lies in the range of 0.9-1.3 ng/ml.
• Specific Activity: 1.11 x 106units/mg
• Reconstitution: Centrifugevial prior to opening. First add sterile distilled water to thevial to fully solubilize the protein to a concentration not less than 100 ug/ml.After complete solubilization of the protein, it can be further diluted toother aqueous solutions.
• storage: Thelyophilized protein, though stable at room temperature for two weeks, is beststored at -20°C.Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: NRG1
• Pathways: ErbB signaling pathway; Glypican1 network; MicroRNAs in cardiomyocyte hypertrophy

Gene Information

• Gene Name: NRG1 neuregulin 1 [ Homosapiens ]
• Synonyms: NRG1; neuregulin 1; GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF;Neuregulin-1, sensory and motor neuron-derived factor isoform;Pro-neuregulin-1, membrane-bound isoform precursor; neu differentiationfactor; neuregulin 1; sensory and motor neuron derived factor; Pro-NRG1;glial growth factor; heregulin, alpha (45kD, ERBB2 p185-activator)
• Gene ID: 3084
• mRNA Refseq: NM_004495
• Protein Refseq: NP_004486
• MIM: 142445
• UniProt ID: Q15491
• Chromosome Location: 8p12
• Function: growth factoractivity; receptor tyrosine kinase binding; Molecular function; ErbB-3 classreceptor binding; transcription cofactor activity; transmembrane receptorprotein tyrosine kinase activator activity