Recombinant Human Alpha-Ketoglutarate-Dependent Dioxygenase AlkB Homolog 3, His-tagged

• Cat.No.: ALKBH3-970H
• Product Overview: Recombinant human ALKBH3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques(1-286 aa).

Specification

• Cat. No.: ALKBH3-970H
• Description: ALKBH3 is a member of the ALKB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates. This protein repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA and its activity is stimulated by ascorbate. ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus.
• Form: Liquid. In 20mM Tris-HClbuffer(pH 8.0) containing 10% glycerol,2mM DTT, 0.1M NaCl.
• Molecular Weight: 37.9 kDa (325aa), confirmed by MALDI-TOF
• Purity: > 95 % by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELDM EEKRRRARVQ GAWAAPVKSQ AIAQPATTAK SHLHQKPGQT WKNKEHHLSD REFVFKEPQQ VVRRAPEPRV IDREGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDITYQQPR LTAWYGELPY TYSRITMEPN PHWHPVLRTL KNRIEENTGH TFNSLLCNLY RNEKDSVDWH SDDEPSLGRC PIIASLSFGA TRTFEMRKKP PPEENGDYTY VERVKIPLDH GTLLIMEGAT QADWQHRVPK EYHSREPRVN LTFRTVYPDP RGAPW
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
• Publication:
  Demethylation of m1A assisted degradation of the signal probe for rapid electrochemical detection of ALKBH3 activity with practical applications (2022)

Gene Information

• Gene Name: ALKBH3 alkB, alkylation repair homolog 3 (E. coli) [Homo sapiens]
• Synonyms: ALKBH3; DEPC-1; DEPC1; FLJ43614; MGC118793; MGC118792; alkB, alkylation repair homolog 3 (E. coli); MGC118790;alpha-ketoglutarate-dependent dioxygenasealkB homolog 3; ABH3;prostate cancer antigen-1; PCA1;EC 1.14.11.; Prostate cancer antigen 1;alkylated DNA repair protein alkB homolog 3
• Gene ID: 221120
• mRNA Refseq: NM_139178
• Protein Refseq: NP_631917
• MIM: 610603
• UniProt ID: Q96Q83
• Chromosome Location: 11p11.2
• Function: 2-oxoglutarate-dependent dioxygenase activity; L-ascorbic acid binding ; DNA-N1-methyladenine dioxygenase activity; cytosine C-5 DNA demethylaseactivity; damaged DNA binding; ferrous iron binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen