||Recombinant human RPE protein, fused to His-tag at N-terminus,was expressed in E. coli and purified byusing conventional chromatography techniques.
||RPE(ribulose-phosphate 3-epimerase) belongs to theribulose-phosphate 3-epimerase family. In enzymology, aribulose-phosphate 3-epimerase is the enzyme that converts D-ribulose5-phosphate into D-xylulose 5-phosphate in Calvin"s reductive pentosephosphate cycle. Hence, this enzyme has one substrate, D-ribulose5-phosphate, and one product, D-xylulose 5-phosphate. This enzymeparticipates in 3 metabolic pathways: pentose phosphate pathway, pentose andglucuronate interconversions, and carbon fixation.
||Liquid.In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 200mM NaCl.
||27.5kDa (252aa),confirmed by MALDI-TOF
||> 95%by SDS-PAGE
||0.5 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMASGCK IGPSILNSDL ANLGAECLRM LDSGADYLHL DVMDGHFVPNITFGHPVVES LRKQLGQDPF FDMHMMVSKP EQWVKPMAVA GANQYTFHLE ATENPGALIK DIRENGMKVGLAIKPGTSVE YLAPWANQID MALVMTVEPG FGGQKFMEDM MPKVHWLRTQ FPSLDIEVDG GVGPDTVHKCAEAGANMIVS GSAIMRSEDP RSVINLLRNV CSEAAQKRSL DR
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoidrepeated freezing and thawing cycles.
||Metabolic pathways; Pentose andglucuronate interconversions; Pentose phosphate pathway