Recombinant Human ATG4 Autophagy Related 4 Homolog B (S. cerevisiae), His-tagged

• Cat.No.: ATG4B-1921H
• Product Overview: Recombinant human ATG4Bprotein, fused to His-tag at C-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Cat. No.: ATG4B-1921H
• Description: ATG4B,also known as cysteine protease ATG4B, is a cysteine protease required forautophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 orGABARAP, allowing the liberation of form I. A subpopulation of form I issubsequently converted to a smaller form (form II).
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF.
• Molecular Weight: 45.4 kDa(401aa) confirmed by MALDI-TOF
• Purity: > 90%by SDS-PAGE
• Concentration: 0.5mg/ml (determined by Bradford assay)
• Sequences of aminoacids: MDAATLTYDTLRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL WFTYRKNFPA IGGTGPTSDT GWGCMLRCGQMIFAQALVCR HLGRDWRWTQ RKRQPDSYFS VLNAFIDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQVLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRTSVPCA GATAFPADSD RHCNGFPAGA EVTNRPSPWRPLVLLIPLRL GLTDINEAYV ETLKHCFMMP QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVEPTDGCFIPDESFH CQHPPCRMSI AELDPSIAVG FFCKTEDDFN DWCQQVKKLS LLGGALPMFE LVEQQPSHLACPDVLNLSLD SSDVERLERF FDSEDEDFEI LSLLEHHHHH H
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Regulationof autophagy

Gene Information

• Gene Name: ATG4B ATG4 autophagy related 4homolog B (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ATG4B
• Synonyms: ATG4B; ATG4 autophagy related 4 homolog B(S. cerevisiae); APG4B; AUTL1; cysteine proteaseATG4B; hAPG4B; autophagin-1; APG4 autophagy 4 homolog B; AUT-like 1 cysteineendopeptidase; autophagy-related protein 4 homolog B; autophagy-relatedcysteine endopeptidase 1; EC 3.4.22.-; APG4 autophagy 4 homolog B (S.cerevisiae); DKFZp586D1822;KIAA0943
• Gene ID: 23192
• mRNA Refseq: NM_013325
• Protein Refseq: NP_037457
• MIM: 611338
• UniProt ID: Q9Y4P1
• Chromosome Location: 2q37.3
• Function: cysteine-type peptidase activity; peptidaseactivity; protein binding