Human ATG4B Knockdown Cell Lysate

• Cat.No.: ATG4B-057HKCL
• Product Overview: WB-validated ATG4B Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 44 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg ATG4B KD HeLa cell lysate
• Protein Families: Protease
• Protein Pathways: Regulation of autophagy
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: ATG4B ATG4 autophagy related 4 homolog B (S. cerevisiae) [ Homo sapiens (human) ]
• Official Symbol: ATG4B
• Synonyms: ATG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); APG4 autophagy 4 homolog B (S. cerevisiae), APG4B; cysteine protease ATG4B; Apg4B; AUTL1; DKFZp586D1822; KIAA0943; hAPG4B; autophagin-1; APG4 autophagy 4 homolog B; AUT-like 1 cysteine endopeptidase; autophagy-related protein 4 homolog B; autophagy-related cysteine endopeptidase 1; APG4B; MGC1353
• Gene ID: 23192
• mRNA Refseq: NM_013325
• Protein Refseq: NP_037457
• MIM: 611338
• UniProt ID: Q9Y4P1