Recombinant Human G Protein-Coupled Receptor 115, His-tagged GPR115-5631H

Recombinant Human G Protein-Coupled Receptor 115, His-tagged


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Recombinant Human G Protein-Coupled Receptor 115, His-tagged

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Cat. No. : GPR115-5631H
Product Overview : RecombinantHuman GPR115 protein was expressed in Chinese HamsterOvary cell line. Ser22-Ala347, with a C-terminal 6-Histag.
Description : GPR115 is a member of the LN-7TM family of adhesion-type7-transmembrane (TM) G-protein coupled receptors (GPCR) that show a longextracellular N-terminus. The 695 amino acid (aa) human GPR115 sequencepredicts a 21 aa signal sequence, a 385 aa N-terminal extracellular domain(ECD), seven TM regions separated by 6-24 aa intracellular and extracellularregions, and a 40 aa cytoplasmic tail. Like other LN-7TM members, the ECDcontains a highly glycosylated mucin-like stalk that is predicted to functionin adhesion. This is followed by a cysteine-rich GPCR proteolytic cleavagesite (GPS).
Source : Chinese HamsterOvary cell line, CHO-derived.
Form : Lyophilized from a0.2 um filtered solution in PBS.
N-terminalSequence : Ser22
MolecularWeight : 37 kDa
Activity : Measuredby the ability of the immobilized protein to support the adhesion of U-87 MGhuman glioblastoma/astrocytoma cells. When 5x104 cells per wellare added to rhGPR115 coated plates (5 μg/mL, 100 μL/well), approximately 60-85%will adhere after 60 minutes at 37°C.
Endotoxin Level : <1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE : 66-100kDa, reducing conditions.
Purity : >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : 200 μg/mLin sterile PBS
Storage : Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 monthsfrom date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C understerile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.
Gene Name : GPR115 G protein-coupledreceptor 115 [ Homo sapiens ]
Official Symbol : GPR115
Synonyms : GPR115; Gprotein-coupled receptor 115; PGR18; probable G-protein coupled receptor115; G-protein coupled receptor PGR18; seven transmembrane helix receptor
Gene ID : 221393
mRNA Refseq : NM_153838
Protein Refseq : NP_722580
MIM : 614268
UniProt ID : Q8IZF3
Chromosome Location : 6p12.3
Function : G-protein coupledreceptor activity; receptor activity; signal transducer activity

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