| Cat. No. : |
GPRASP1-5633H |
| Description : |
GPRASP1 was isolated in a screen to identify proteins in micethat copurify with myostatin (GDF-8), a potent negative regulator of skeletalmuscle mass. The GPRASP1 cDNA encodes a 571amino acid protein that contains a 29 amino acid signal sequence and iscomprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin,two tandem Kunitz, and netrin. Two related human proteins which contain thesame domain structure are called WFIKKN (based on the presence and order ofthese conserved domains) and WFIKKNRP (WFIKKN-related protein). |
| Source : |
Mammalian cells |
| Form : |
Lyophilized from a0.2 um filtered solution in PBS with BSA as a carrier protein. |
| N-terminalSequence : |
Leu35 |
| MolecularWeight : |
60.7 kDa |
| Activity : |
Measuredby its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenousleukemia cells. The ED50 for this effect is typically 0.03-0.15 μg/mL, in thepresence of 40 ng/mL of rmGDF-8. Measured by its ability to inhibit trypsin cleavageof a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 valueis approximately 1.5 nM, as measured in a reaction mixture containing 1.0 nMtrypsin,10 mM ES002, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
75-80kDa, reducing conditions. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
10 μg/mLin sterile PBS containing at least 0.1% human or bovine serum albumin |
| Storage : |
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 monthsfrom date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C understerile conditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution. |
