Active Recombinant Mouse Interleukin 3 Receptor, Alpha Chain, Fc-tagged

• Cat.No.: Il3ra-5661M
• Product Overview: RecombinantMouse Il3ra protein was expressed in Spodopterafrugiperdawith the Fc part of Human IgG1 Pro100-Lys330 (C-terminus) and MouseIl3ra Ser17-Lys331 (N-terminus).

Specification

• Species: Mouse
• Source: S.frugiperda
• Tag: Fc
• Protein Length: 17-331 a.a.
• Description: Il3ra isa pleiotropic cytokine produced primarily by activated T cells or mast cells.Il3ra stimulates the proliferation and differentiation of hemopoietic cellsincluding the pluripotent hematopoietic stem cells as well as various lineage-committedcells. The biological effects of Il3ra on the various cell types are mediatedby the binding of Il3ra to specific cell surface receptor complexes. Thefunctional high-affinity IL-3 receptor is a heterodimer consisting of aligand binding α subunit and the β subunit. The α subunit alone binds Il3rawith low affinity.
• Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS.
• N-terminalSequence: Ser17
• MolecularWeight: 61.0 kDa (monomer)
• Activity: Measuredby its ability to inhibit the IL-3-induced proliferation of NFS-60 mouse myeloidcells. The ED50 for this effect is typically 0.03-0.15 μg/mL in the presenceof 2.5 μg/mL Recombinant Mouse IL-3 Rβ Fc Chimera and 2 ng/mL of RecombinantMouse Il3ra.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 70-80kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Il3ra interleukin 3 receptor,alpha chain [ Mus musculus ]
• Official Symbol: Il3ra
• Synonyms: Il3ra; interleukin 3receptor, alpha chain; CD123; SUT-1; CDw123;interleukin-3 receptor subunit alpha; IL-3RA; IL-3R-alpha; Il-3 alpha subunit;IL-3R subunit alpha; IL-3 receptor alpha chain; IL-3 receptor subunit alpha;IL-3 receptor alpha SP2 isoform; interleukin-3 receptor class II alpha chain
• Gene ID: 16188
• mRNA Refseq: NM_008369
• Protein Refseq: NP_032395
• UniProt ID: P26952
• Chromosome Location: 14
• Pathway: Apoptosis;Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; IL-3Signaling Pathway; Jak-STAT signaling pathway
• Function: cytokine receptoractivity; receptor activity