Active Recombinant Human Klotho Beta, His-tagged
| Cat.No. : | KLB-5671H |
| Product Overview : | Recombinant Human KLBprotein was expressed in Murine myeloma cell line with a C-terminal 10-Histag. Phe53-Leu997. |
- Specification
- Gene Information
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| Cat. No. : | KLB-5671H |
| Description : | KLB, a divergentstructural member of the glycosidase I superfamily, is expressed primarily inthe liver and pancreas, with lower expression in adipose tissue. Like Klotho,KLB facilitates binding between FGF19 subfamily members and their receptorsvia formation of a ternary complex. The KLB mediated interaction of humanFGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulatesbile acid synthesis by controlling the secretion of two key bile acidsynthase genes, Cyp7a1 and Cyp8b1. |
| Source : | Mammalian cells |
| Form : | Supplied as a 0.2 μmfiltered solution in PBS, Glycerol and EDTA. |
| N-terminalSequence : | Phe53 |
| Molecular Weight : | 110 kDa |
| Activity : | Measuredby its binding ability in a functional ELISA. When 2 μg/mL (100 μL/well) ofrhKLB was immobilized onto Ms x hKLB coated plate, the concentration ofrhFGF-21 that produces 50% of the optimal binding response was found to beapproximately 3-18 ng/mL. |
| Endotoxin Level : | <1.0 EUper 1 μg of the protein by the LAL method. |
| SDS-PAGE : | 126 kDa,reducing conditions. |
| Purity : | >95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Storage : | Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after opening. |
| Gene Name : | KLB klotho beta [ Homosapiens ] |
| Official Symbol : | KLB |
| Synonyms : | KLB; klotho beta;BKL; MGC142213; beta-klotho; betaKlotho; klotho beta like; klotho beta-likeprotein; EC 3.2.18; OTTHUMP00000158728 |
| Gene ID : | 152831 |
| mRNA Refseq : | NM_175737 |
| Protein Refseq : | NP_783864 |
| MIM : | 611135 |
| UniProt ID : | Q86Z14 |
| Chromosome Location : | 4p14 |
| Pathway : | Downstream signalingof activated FGFR; FGF signaling pat; FGFR ligand binding and activation;FRS2-mediated cascade; IRS-mediated signalling; IRS-related events; Insulinreceptor signalling cascade; Klotho-mediated ligand binding; Negativeregulation of FGFR signaling; PI-3K cascade; PI3K Cascade; PhospholipaseC-mediated cascade; SHC-mediated cascade; Signal Transduction; Signaling byFGFR |
| Function : | catalytic activity;cation binding; fibroblast growth factor binding; hydrolase activity |
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