Active Recombinant Human KLB protein, His-tagged, Low Endotoxin

• Cat.No.: KLB-539H
• Product Overview: Active Recombinant Human KLB protein(Q86Z14)(Met30-Thr983) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 30-983 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human Beta Klotho at 2μg/ml (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLB klotho beta [ Homo sapiens ]
• Official Symbol: KLB
• Synonyms: KLB; klotho beta; beta-klotho; betaKlotho; klotho beta like; klotho beta-like protein; BKL; MGC142213
• Gene ID: 152831
• mRNA Refseq: NM_175737
• Protein Refseq: NP_783864
• MIM: 611135
• UniProt ID: Q86Z14