Recombinant Human KLB protein, His-tagged

• Cat.No.: KLB-539H
• Product Overview: Recombinant Human KLB protein(Q86Z14)(Met30-Thr983), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met30-Thr983
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLB klotho beta [ Homo sapiens ]
• Official Symbol: KLB
• Synonyms: KLB; klotho beta; beta-klotho; betaKlotho; klotho beta like; klotho beta-like protein; BKL; MGC142213
• Gene ID: 152831
• mRNA Refseq: NM_175737
• Protein Refseq: NP_783864
• MIM: 611135
• UniProt ID: Q86Z14