Active Recombinant Mouse Neuroblastoma, Suppression Of Tumorigenicity 1, His-tagged

• Cat.No.: Nbl1-5690M
• Product Overview: Recombinant Mouse Nbl1protein was expressed in Murine myeloma cell line with a C-terminal Ile and10-His tag. Ala17-Asp178.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 17-178 a.a.
• Description: Nbl1 was initiallyidentified as a gene whose expression is downregulated in srctransformed ratfibroblasts. Nbl1 has now been shown to be a prototypical member of the Nbl1family of secreted glycoproteins that are putative BMP antagonists. Nbl1family members share a cysteine-rich domain that is structurally related tothe cysteine knot motif found in TGFβ superfamily ligands. There are at leastfive mouse Nbl1 family members, including Nbl1, Gremlin/DRM, Cer1, Dante andPRDC. Additional Nbl1 family members include Xenopus Cerberus, chick Caronte,and C. elegans CeCan 1.
• Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Ala17
• Molecular Weight: 18.9 kDa
• Activity: Measuredby its ability to bind rhBMP4 in a functional ELISA. Immobilized rmNbl1 at 2 μg/mL (100 μL/well) can bind rhBMP4 with a linearrange of 0.1-10 ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 27-30kDa, reducing conditions.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 10 μg/mLin sterile PBS that containing at least 0.1% human or bovine serum albumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: Nbl1 neuroblastoma, suppressionof tumorigenicity 1 [ Mus musculus ]
• Official Symbol: Nbl1
• Synonyms: Nbl1; neuroblastoma,suppression of tumorigenicity 1; DAN; NO3; Dana; D4H1S1733E; MGC123430;neuroblastoma suppressor of tumorigenicity 1; N03; zinc finger protein DAN
• Gene ID: 17965
• mRNA Refseq: NM_008675
• Protein Refseq: NP_032701
• UniProt ID: Q61477
• Chromosome Location: 4 D3; 4 70.0 cM