Cat. No. : |
Hgf-734M |
Product Overview : |
Recombinant Mouse Hgfprotein was expressed in Murine myeloma cell line. Gln33-Arg495 (alpha) &Val496-Leu728 (beta). |
Description : |
Hgf, also known asScatter Factor and Hepatopoietin A, is a pleiotropic protein in thePlasminogen subfamily of S1 peptidases. It is a multidomain molecule thatincludes an N-terminal PAN/APPLE-like domain, four Kringle domains, and aserine proteinase-like domain that has no detectable protease activity. MouseHgf is secreted as an inactive 728 amino acid (aa) single chain propeptide. |
Source : |
Murine myeloma cellline, NS0-derived. |
Form : |
Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein. |
N-terminalSequence : |
Gln33 predicted(alpha) & Val496 (beta) |
Molecular Weight : |
53.4 kDa (α-chain),26 kDa (β-chain) |
Activity : |
Measuredin a cell proliferation assay using IMCD3 mouse epithelial cells. The ED50for this effect is typically 10-30 ng/mL. Measured by its ability to bindRecombinant Mouse Met Fc Chimera in a functional ELISA with an estimated KD< 0.2 nM. |
Endotoxin Level : |
<1.0 EUper 1 μg of the protein by the LAL method. |
SDS-PAGE : |
60 kDaand 33 kDa, reducing conditions. |
Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution : |
10 μg/mLin sterile PBS that containing at least 0.1% human or bovine serum albumin. |
Storage : |
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution. |