Active Recombinant Mouse Hepatocyte Growth Factor

• Cat.No.: Hgf-734M
• Product Overview: Recombinant Mouse Hgfprotein was expressed in Murine myeloma cell line. Gln33-Arg495 (alpha) &Val496-Leu728 (beta).

Specification

• Cat. No.: Hgf-734M
• Description: Hgf, also known asScatter Factor and Hepatopoietin A, is a pleiotropic protein in thePlasminogen subfamily of S1 peptidases. It is a multidomain molecule thatincludes an N-terminal PAN/APPLE-like domain, four Kringle domains, and aserine proteinase-like domain that has no detectable protease activity. MouseHgf is secreted as an inactive 728 amino acid (aa) single chain propeptide.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Gln33 predicted(alpha) & Val496 (beta)
• Molecular Weight: 53.4 kDa (α-chain),26 kDa (β-chain)
• Activity: Measuredin a cell proliferation assay using IMCD3 mouse epithelial cells. The ED50for this effect is typically 10-30 ng/mL. Measured by its ability to bindRecombinant Mouse Met Fc Chimera in a functional ELISA with an estimated KD< 0.2 nM.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 60 kDaand 33 kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 10 μg/mLin sterile PBS that containing at least 0.1% human or bovine serum albumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: Hgf hepatocyte growth factor [ Musmusculus ]
• Official Symbol: Hgf
• Synonyms: Hgf; hepatocytegrowth factor; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik; SF; scatter factor;hepatopoeitin-A; Scatter factor
• Gene ID: 15234
• mRNA Refseq: NM_010427
• Protein Refseq: NP_034557
• UniProt ID: Q08048
• Chromosome Location: 5 A2-A3; 5 4.0 cM
• Pathway: Cytokine-cytokinereceptor interaction; Focal Adhesion; Focal adhesion; Hemostasis; Malaria;Melanoma; Pathways in cancer; Platelet activation; Platelet degranulation;Renal cell carcinoma; Response to elevated platelet cytosolic Ca2+;Signaling of Hepatocyte Growth Factor Receptor
• Function: catalytic activity;growth factor activity; protein heterodimerization activity; serine-typeendopeptidase activity