Recombinant Human Platelet Endothelial Aggregation Receptor 1, His-tagged

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Recombinant Human Platelet Endothelial Aggregation Receptor 1, His-tagged

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Cat. No. : PEAR1-498H
Product Overview : Recombinant Human PEAR1protein was expressed in CHO with a C-terminal 6-His tag. Leu21-Ser754.
Description : PEAR1 is a 150 kDatype I transmembrane protein and member of the MEGF family of proteins. HumanPEAR1 is synthesized as a 1037 amino acid (aa) precursor that contains a 20aa signal sequence, a 735 aa extracellular domain (ECD), a 21 aatransmembrane region, and a 261 aa cytoplasmic region.
Source : Chinese HamsterOvary cell line, CHO-derived.
Form : Lyophilized from a0.2 μm filtered solution in PBS.
N-terminalSequence : Leu21
Molecular Weight : 78.3 kDa
Activity : Measuredby the ability of the immobilized protein to support the adhesion of thrombinactivated human platelets. When 5 x 106 cells per well are addedto rhPEAR1 coated plates (1 μg/mL, 100 μL/ well), approximately 50%-70% willadhere after 1 hour at 37°C.
Endotoxin Level : <1.0 EUper 1 μg of the protein by the LAL method.
SDS-PAGE : 120-130kDa, reducing conditions.
Purity : >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : 200 μg/ mLin PBS
Storage : Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.
Gene Name : PEAR1 platelet endothelialaggregation receptor 1 [ Homo sapiens ]
Official Symbol : PEAR1
Synonyms : PEAR1; plateletendothelial aggregation receptor 1; JEDI; MEGF12; FLJ00193; Hpear1; multipleEGF-like-domains 12; multiple EGF-like domains protein 12; multiple epidermalgrowth factor-like domains protein 12; OTTHUMP00000038718
Gene ID : 375033
mRNA Refseq : NM_001080471
Protein Refseq : NP_001073940
MIM : 610278
UniProt ID : Q5VY43
Chromosome Location : 1q23.1
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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