"PEAR1" Related Products

Recombinant Human Platelet Endothelial Aggregation Receptor 1, His-tagged

Cat. No.: PEAR1-498H
Product Overview: Recombinant Human PEAR1protein was expressed in CHO with a C-terminal 6-His tag. Leu21-Ser754.
Description: PEAR1 is a 150 kDatype I transmembrane protein and member of the MEGF family of proteins. HumanPEAR1 is synthesized as a 1037 amino acid (aa) precursor that contains a 20aa signal sequence, a 735 aa extracellular domain (ECD), a 21 aatransmembrane region, and a 261 aa cytoplasmic region.
Source: Chinese HamsterOvary cell line, CHO-derived.
Form: Lyophilized from a0.2 μm filtered solution in PBS.
N-terminalSequence: Leu21
Molecular Weight: 78.3 kDa
Activity: Measuredby the ability of the immobilized protein to support the adhesion of thrombinactivated human platelets. When 5 x 106 cells per well are addedto rhPEAR1 coated plates (1 μg/mL, 100 μL/ well), approximately 50%-70% willadhere after 1 hour at 37°C.
Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
SDS-PAGE: 120-130kDa, reducing conditions.
Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: 200 μg/ mLin PBS
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.
Gene Name: PEAR1 platelet endothelialaggregation receptor 1 [ Homo sapiens ]
Official Symbol: PEAR1
Synonyms: PEAR1; plateletendothelial aggregation receptor 1; JEDI; MEGF12; FLJ00193; Hpear1; multipleEGF-like-domains 12; multiple EGF-like domains protein 12; multiple epidermalgrowth factor-like domains protein 12; OTTHUMP00000038718
Gene ID: 375033
mRNA Refseq: NM_001080471
Protein Refseq: NP_001073940
MIM: 610278
UniProt ID: Q5VY43
Chromosome Location: 1q23.1

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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