Active Recombinant Human Wingless-Type MMTV Integration Site Family, Member 4

• Cat.No.: WNT4-516H
• Product Overview: Recombinant Human WNT4protein was expressed in Chinese Hamster Ovary cell line. Ser23-Arg351.

Specification

• Cat. No.: WNT4-516H
• Description: WNT4 is a 38-42kDamember of the Wnt family of secreted glycoproteins, which act as shortrange signalingmolecules via Frizzled receptors and a cascade of intracellular signals invertebrate embryogenesis. Human WNT4 is synthesized as a 351 amino acid (aa)precursor with a 22 aa signal sequence and a 329 aa mature chain. The maturechain contains two potential sites for Nlinked glycosylation. Relative toother members of the Wnt family, WNT4 contains 83 conserved aa, including 21cysteines.
• Source: Chinese HamsterOvary cell line, CHO-derived.
• Form: Lyophilized from a0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrierprotein.
• N-terminalSequence: Ser23
• Molecular Weight: 39.1 kDa
• Activity: Measured by itsability to induce alkaline phosphatase production by MC3T3E1 mouseosteoblastic cells. The typical ED50 for this effect is 25-100 ng/mL.
• Endotoxin Level: < 1.0 EU per 1 μgof the protein by the LAL method.
• SDS-PAGE: 42 - 80 kDa,reducing conditions.
• Purity: > 60%, by SDSPAGEunder reducing conditions and visualized by silver stain.
• Reconstitution: 50 μg/mL in PBS.
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: WNT4 wingless-type MMTVintegration site family, member 4 [ Homo sapiens ]
• Official Symbol: WNT4
• Synonyms: WNT4; wingless-typeMMTV integration site family, member 4; WNT-4; SERKAL; protein Wnt-4; UNQ426/PRO864
• Gene ID: 54361
• mRNA Refseq: NM_030761
• Protein Refseq: NP_110388
• MIM: 603490
• UniProt ID: P56705
• Chromosome Location: 1p36.23-p35.1
• Pathway: Basal cell carcinoma;Class B/2 (Secretin family receptors); DNA damage response (only ATMdependent); GPCR ligand binding; HTLV-I infection; Hedgehog signaling pathway;Melanogenesis; Pathways in cancer; Signal Transduction; Signaling by GPCR; WntSignaling Pathway; Wnt Signaling Pathway NetPath; Wnt Signaling Pathway andPluripotency; Wnt signaling pathway
• Function: G-protein coupledreceptor binding; extracellular matrix structural constituent; frizzledbinding; transcription corepressor activity