Recombinant Human ADAM Metallopeptidase With Thrombospondin type 1 Motif, 15, His-tagged


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Recombinant Human ADAM Metallopeptidase With Thrombospondin type 1 Motif, 15, His-tagged

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Cat. No. : ADAMTS15-528H
Product Overview : Recombinant Human ADAMTS15protein was expressed in Chinese Hamster Ovary cell line with a C-terminal 10-Histag. Gly18-Cys682 (pro) & Phe213-Cys682 (mature).
Description : ADAMTS (adisintegrin and metalloproteinase with thrombospondin motifs) is a group ofnineteen secreted zinc binding metalloproteases that is involved indevelopment, inflammation, angiogenesis and several pathological conditions.They are synthesized as zymogens which have a prodomain that is removed byfurinlike protein convertases. The mature secreted enzymes consist ofcatalytic, disintegrinlike, type I thrombospondin (TSP) motif, cysteine rich,and spacer domains followed by zero to fourteen additional TSP motifs.
Source : Chinese HamsterOvary cell line, CHO-derived.
Form : Supplied as a 0.2 μmfiltered solution in MES and NaCl.
N-terminalSequence : Gly18 & Phe213
Molecular Weight : 74 kDa (pro), 53 kDa(mature)
Activity : Measured by itsability to cleave rhAggrecan. Cleavage of 1 μg of rhAggrecan is > 90%, asmeasured under the described conditions.
Endotoxin Level : < 1.0 EU per 1 μgof the protein by the LAL method.
SDS-PAGE : 85 kDa and 65 kDa,reducing conditions.
Purity : > 80%, by SDSPAGEunder reducing conditions and visualized by silver stain.
Reconstitution : 100 μg/mL in sterilePBS.
Storage : Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt,-20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditionsafter opening.
Gene Name : ADAMTS15 ADAM metallopeptidasewith thrombospondin type 1 motif, 15 [ Homo sapiens ]
Official Symbol : ADAMTS15
Synonyms : ADAMTS15; ADAMmetallopeptidase with thrombospondin type 1 motif, 15; Adisintegrin and metalloproteinase with thrombospondin motifs 15; ADAM-TS15;ADAMTS-15; ADAM-TS 15; metalloprotease disintegrin 15 with thrombospondindomains; a disintegrin-like and metalloprotease (reprolysin type) withthrombospondin type 1 motif, 15, preproprotein
Gene ID : 170689
mRNA Refseq : NM_139055
Protein Refseq : NP_620686
MIM : 607509
UniProt ID : Q8TE58
Chromosome Location : 11q25
Function : metal ion binding;metalloendopeptidase activity; peptidase activity; zinc ion binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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