Active Recombinant Mouse Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1, His-tagged

• Cat.No.: Aimp1-549M
• Product Overview: Recombinant Mouse Aimp1protein was expressed in E. coli with a N-terminal Met and 6-His tag. Ala2-Lys310.

Specification

• Cat. No.: Aimp1-549M
• Description: Aimp1 is a 23 kDa polypeptideoriginally identified in the supernatant of TNFα treated Meth A fibrosarcomacells. In addition to EMAPI/S100C and EMAPIII/VEGF, Aimp1 was one of threemolecules presumed to enhance TNFαinduced tissue factor expression on endothelialcells. ProEMAPII, otherwise known as p43, is the 310 amino acid (aa)precursor to EMAPII. It is considered to have widespread, if not ubiquitousexpression, and is found both intraand extracellularly. ProAimp1 contains no canonicalsignal sequence. It does possess a 144 aa N-terminus that is characterized bythe presence of two coiled coil regions (aa 6-77) and two heparinbinding motifs(aa 71-73 and 120-122). The C-terminal 166 aa are synonymous with Aimp1.
• Source: E. coli-derived
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Met
• Molecular Weight: 34.7 kDa
• Activity: Measured by itsability to induce TNFα secretion by RAW 264.7 mouse macrophages. WhenrmEMAPII is coated, it will induce TNFα production by RAW 264.7 cells in the presenceof Polymyxin B (20 μg/mL) with an ED50 range of 3-10 μg/mL.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• Purity: > 95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in sterile PBS.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Aimp1 aminoacyl tRNA synthetasecomplex-interacting multifunctional protein 1 [ Mus musculus ]
• Official Symbol: Aimp1
• Synonyms: Aimp1; aminoacyltRNA synthetase complex-interacting multifunctional protein 1; p43;Emap2; Scye1; EMAPII; AIMP1/p43; 9830137A06Rik; ARS inducible multifunctionalprotein 1; endothelial monocyte activating polypeptide 2; multisynthasecomplex auxiliary component p43; small inducible cytokine subfamily E, member1; multisynthetase complex auxiliary component p43
• Gene ID: 13722
• mRNA Refseq: NM_007926
• Protein Refseq: NP_031952
• Chromosome Location: 3; 3 H2
• Pathway: Cytosolic tRNAaminoacylation; Gene Expression; mRNA processing; tRNA Aminoacylation
• Function: cell surfacebinding; protein homodimerization activity; tRNA binding