"Bmp10" Related Products


Recombinant Mouse Bone Morphogenetic Protein 10

Cat. No.: Bmp10-575M
Product Overview: Recombinant Mouse Bmp10protein was expressed in Chinese Hamster Ovary cell line. Asn314-Arg421.
Description: Bmp10, along with BMP9,GDF5, 6, and 7, belongs to a subgroup of TGFβ superfamily proteins thatsignal through heterodimeric complexes composed of type I and type II BMPreceptors. Proteolytic removal of the propeptide from the 60 kDa proproteinyields a 12 kDa mature Bmp10 which forms disulfidelinked nonglycosylatedhomodimers.
Source: Chinese HamsterOvary cell line, CHO-derived.
Form: Lyophilized from a0.2 μm filtered solution in PBS.
N-terminalSequence: Asn314
Molecular Weight: 12.1 kDa (monomer)
Activity: Measured by itsability to induce alkaline phosphatase production by MC3T3-E1 mouseosteoblastic cells. The ED50 for this effect is typically 15-75 ng/mL.
Endotoxin Level: < 1.0 EU per 1 μgof the protein by the LAL method.
SDS-PAGE: 10 kDa,reducing conditions.
Purity: > 95%, by SDSPAGEunder reducing conditions and visualized by silver stain.
Reconstitution: 250 μg/mL in 4 mMHCl containing at least 0.1% human or bovine serum albumin.
Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
Gene Name: Bmp10 bone morphogeneticprotein 10 [ Mus musculus ]
Official Symbol: Bmp10
Synonyms: Bmp10; bonemorphogenetic protein 10; BMP-10
Gene ID: 12154
mRNA Refseq: NM_009756
Protein Refseq: NP_033886
Chromosome Location: 6; 6 D2
Function: cytokine activity;growth factor activity; receptor serine/threonine kinase binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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