| Cat. No. : |
Gas1-605M |
| Description : |
Gas1 isone of six structurally unrelated proteins that were identified by theirincreased expression in growth-arrested cells relative to activelyproliferating cells. Following mitogenic stimulation, Gas1 expression is transcriptionallysuppressed by c-Myc as cells transit from G 0 to G1 phases of the cell cycle.Overexpression of Gas1 prevents S phase entry and DNA synthesis. Gas-mediatedblockade of the cell cycle is p53-dependent but does not require thetransactivating domain of p53. The mouse Gas1 cDNA encodes a 343 amino acid(aa) precursor that includes a 38 aa signal sequence, a 277 aa matureprotein, and a 28 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-richregions as well as an RGD sequence. |
| Source : |
Mammalian cells |
| Form : |
Lyophilized from a0.2 um filtered solution in PBS. |
| N-terminalSequence : |
Leu39 & Arg43 |
| MolecularWeight : |
29.8 kDa |
| Activity : |
Measuredby its binding ability in a functional ELISA. Immobilized rhGas1 at 1 μg/mL(100 μL/well) can bind rmArtemin at a linear range of 0.1-10 ng/mL. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
40 kDa,reducing conditions. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstitute at100μg/mL in PBS. |
| Storage : |
Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution. |
