Active Recombinant Human Growth Arrest-Specific 6, His-tagged

• Cat.No.: GAS6-613H
• Product Overview: RecombinantHuman GAS6 protein was expressed in Murinemyeloma cell line. Ala49-Ala678, with a C-terminal 6-Histag.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 49-678 a.a.
• Description: GAS6 is a multimodular protein that is upregulated by awide variety of cell types in response to growth arrest. GAS6 and the structurally related Protein S are vitamin Kdependentand have an extensively γ-carboxylated N-terminal Gla domain, four EGF-like repeats,and a C-terminal region with homology to steroid hormone binding globulin(SHBG). Human GAS6 is a 75 kDa proteinthat shares 77%-79% aa sequence identity with mouse and rat GAS6, and 43% aa identity with human protein S (over the regionexpressed). GAS6 binds and induces signaling through the receptortyrosine kinases Axl, Dtk, and Mer. Human GAS6 interactswith both mouse and rat orthologs of these receptors.
• Form: Lyophilized from a0.2 μm filtered solution in Tris, NaCl and Citrate.
• N-terminalSequence: Ala49
• MolecularWeight: 70.5 kDa
• Activity: Measuredin a cell proliferation assay using T98G human glioblastoma cells. The ED50 forthis effect is typically 100-400 ng/mL. Also measured by its ability to bindRecombinant Human Axl Fc Chimera in a functional ELISA.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 70-85kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in sterile H2O.
• Storage: Use a manual defrost freezer and avoid repeated freezethawcycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: GAS6 growth arrest-specific 6 [ Homosapiens ]
• Official Symbol: GAS6
• Synonyms: GAS6; growtharrest-specific 6; AXSF; AXLLG; AXLstimulatory factor; AXL receptor tyrosine kinase ligand
• Gene ID: 2621
• mRNA Refseq: NM_000820
• Protein Refseq: NP_000811
• MIM: 600441
• UniProt ID: Q14393
• Chromosome Location: 13q34
• Pathway: Cell surfaceinteractions at the vascular wall; Gamma-carboxylation of protein precursors;Gamma-carboxylation; Platelet activation; Hemostasis; Metabolism of proteins;Platelet degranulation; Post-translational protein modification; Removal ofaminoterminal propeptides from gamma-carboxylated proteins; Transport ofgamma-carboxylated protein precursors from the endoplasmic reticulum to theGolgi apparatus
• Function: calcium ion binding;receptor agonist activity; receptor binding; receptor tyrosine kinase binding