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Active Recombinant Mouse Granzyme D, His-tagged

Cat.No. : Gzmd-1967M
Product Overview : RecombinantMouse Gzmd protein was expressed in Murine myeloma cell line. Ile21-Leu252,with a C-terminal 10-His tag.
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Cat. No. : Gzmd-1967M
Description : Gzmd is amember of the granzyme family of the serine proteases found specifically inthe cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer(NK) cells. Gzmd'sfunctions are largely unknown. However, its selective expression in primaryand functional hematopoietic stromal lines correlates with capacity ofsupporting growth of a pre-T lymphoma clone. Together with Granzymes E, F andG, it is regulated through pregnancy and by IL-2 and IL-15 in granulatedmetrial gland cells. The human or rat counterpart of mouse Gzmd has notbeen found. Like other granzymes, mouse Gzmd is notsecreted as a zymogen but stored as a fully processed and activated enzyme inthe cytoplasmic granules of CTL. It is synthesized as a 252 amino acidprecursor with an 18 amino acid signal peptide and a 2 amino acid propeptide.The mature protein (residues 21-252) is expressed and purified.
Source : Mammalian cells
Form : Supplied as a 0.2 umfiltered solution in HEPES and NaCl.
N-terminalSequence : Ile21 & His25
MolecularWeight : 27 kDa
Activity : Measuredby its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzylester (Suc-FLF-SBzl), in the presence of 5, 5'-Dithiobis (2-nitrobenzoic acid)(DTNB). The specific activity is > 50 pmoles/min/μg, as measured under thedescribed conditions.
Endotoxin Level : <1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE : 42 kDa,reducing conditions.
Purity : >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : Reconstituteat 100μg/mL in sterile 25 mM HEPES and 200 mM NaCl, pH 7.5.
Storage : Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months, -20 to -70°C understerile conditions after opening.
Gene Name : Gzmd granzyme D [ Musmusculus ]
Official Symbol : Gzmd
Synonyms : Gzmd; granzyme D; CCP5;Ctla5; Ctla-5
Gene ID : 14941
mRNA Refseq : NM_010372
Protein Refseq : NP_034502
UniProt ID : P11033
Chromosome Location : 14
Pathway : Activation;Apoptosis; Intrinsic Pathway for Apoptosis
Function : hydrolase activity;peptidase activity; serine-type peptidase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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