| Cat. No. : |
Gzmg-1968M |
| Description : |
Gzmg is amember of the Granzyme family of the serine proteases found specifically inthe cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer(NK) cells. Together with Gzmg, E and F, it isregulated through pregnancy and by IL-2 and IL-15 in granulated metrial glandcells. Human or rat counterpart of mouse Gzmg has notbeen found. Like other Granzymes, mouse Gzmg is notsecreted as a zymogen but stored as a fully processed and activated enzyme inthe cytoplasmic granules of CTL. It is synthesized as a 248 amino acid precursorwith a 18 amino acid signal peptide and a 2 amino acid propeptide. The matureprotein (residues 21-248) is expressed and purified. |
| Source : |
Spodopterafrugiperda,Sf 21 (baculovirus) derived. |
| Form : |
Supplied as a 0.2 umfiltered solution in Tris and NaCl. |
| N-terminalSequence : |
Ile21 |
| MolecularWeight : |
27 kDa |
| Activity : |
Measuredby its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzylester (Suc-FLF-SBzl), in the presence of 5, 5'-Dithiobis (2-nitrobenzoic acid)(DTNB). The specific activity is > 150 pmoles/min/μg, as measured underthe described conditions. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
35 kDa,reducing conditions. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Storage : |
Use a manualdefrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months, -20 to -70°C understerile conditions after opening. |
