Active Recombinant Mouse Kell Blood Group, His-tagged

• Cat.No.: Kel-1976M
• Product Overview: RecombinantMouse Kel protein was expressed in Murine myeloma cell line. Phe50-Trp713,with an N-terminal 7-His tag.

Specification

• Cat. No.: Kel-1976M
• Description: Kel islinked through a single disulfide bond to XK, a putative membranetransporter. The two proteins constitute the Kel bloodgroup antigens. Kel is a zinc endopeptidase of the neprilysin(NEP) family which also includes endothelin converting enzymes (ECE-1 and ECE-2),PEX, XCE, DINE and several NEP-like proteins. It has been shown to cleave bigendothelin-3 (ET-3) at Trp21-Ile22, yielding ET-3, and to a much lesserextent, big ET-1 and big ET-2 at Trp21-Val22, yielding ET-1 and ET-2. Severaldifferent molecular defects cause the Kel null phenotype,which has no obvious clinical outcome.
• Source: Mammalian cells
• Form: Supplied as a 0.2 umfiltered solution in Tris, NaCl and ZnCl2.
• N-terminalSequence: His
• MolecularWeight: 76 kDa
• Activity: Measuredby its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp)-OH.The specific activity is > 0.2 pmoles/min/μg, as measured under thedescribed conditions.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 106 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freezethawcycles. 6months from date of receipt, -20 to -70°C as supplied. 3 months, -20 to -70°Cunder sterile conditions after opening.

Gene Information

• Gene Name: Kel Kell blood group [ Musmusculus ]
• Official Symbol: Kel
• Synonyms: Kel; Kell bloodgroup; Ece3;CD238; kell blood group glycoprotein homolog; Kell protein; Kel blood groupprotein; Kell blood group, metallo-endopeptidase
• Gene ID: 23925
• mRNA Refseq: NM_032540
• Protein Refseq: NP_115929
• UniProt ID: Q9EQF2
• Chromosome Location: 6
• Function: endopeptidase activity;hydrolase activity; metal ion binding; metalloendopeptidase activity;metallopeptidase activity; peptidase activity