| Cat. No. : | AMH-383H |
| Product Overview : | RecombinantHuman AMH protein was expressed in E. coli. Ala453-Arg560. |
| Description : | AMH is atissue-specific TGF-β superfamily growth factor. Its expression is restrictedto the Sertoli cells of fetal and postnatal testis, and to the granulosacells of postnatal ovary. The human MIS gene encodes a 553 amino acid residue(aa) prepropeptide containing a signal a sequence (1-24), a proregion (25-455),and the carboxylterminal bioactive protein (44-6553). MIS is synthesized andsecreted as a disulfide-linked homodimeric pro-protein. Proteolytic cleavageis required to generate the N-terminal pro-region and the C-terminal bioactiveprotein, which remain associated in a noncovalent complex. |
| Source : | E. coli |
| Form : | Lyophilizedfrom a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein. |
| N-terminalSequence : | Ala453 |
| MolecularWeight : | 11.7 kDa (monomer) |
| Activity : | Measuredby its ability to inhibit growth of OVCAR-3 human ovarian carcinoma cells. Measuredby its binding ability in a functional ELISA. Immobilized rhMIS at 3 μg/Ml (100μL/well) will bind rrMIS RII/Fc Chimera with a linear range of 1.6-100 ng/mL. |
| Endotoxin Level : | <1.0EU per 1μg of the protein by the LAL method. |
| Purity : | >97%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : | Reconstituteat 10μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin. |
| Storage : | Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution. |
| Gene Name : | AMH anti-Mullerian hormone [ Homosapiens ] |
| Official Symbol : | AMH |
| Synonyms : | AMH; anti-Mullerianhormone; MIF;MIS; muellerian-inhibiting factor; Mullerian inhibiting factor; Mullerianinhibiting substance; muellerian-inhibiting substance |
| Gene ID : | 268 |
| mRNA Refseq : | NM_000479 |
| Protein Refseq : | NP_000470 |
| MIM : | 600957 |
| UniProt ID : | P03971 |
| Chromosome Location : | 19p13.3 |
| Pathway : | Cytokine-cytokine receptor interaction; ALK2signaling events; TGF-beta signaling pathway |
| Function : | growth factor activity; hormone activity; receptorbinding; transforming growth factor beta receptor binding |
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