| Cat. No. : |
AMH-383H |
| Description : |
AMH is atissue-specific TGF-β superfamily growth factor. Its expression is restrictedto the Sertoli cells of fetal and postnatal testis, and to the granulosacells of postnatal ovary. The human MIS gene encodes a 553 amino acid residue(aa) prepropeptide containing a signal a sequence (1-24), a proregion (25-455),and the carboxylterminal bioactive protein (44-6553). MIS is synthesized andsecreted as a disulfide-linked homodimeric pro-protein. Proteolytic cleavageis required to generate the N-terminal pro-region and the C-terminal bioactiveprotein, which remain associated in a noncovalent complex. |
| Source : |
E. coli |
| Form : |
Lyophilizedfrom a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein. |
| N-terminalSequence : |
Ala453 |
| MolecularWeight : |
11.7 kDa (monomer) |
| Activity : |
Measuredby its ability to inhibit growth of OVCAR-3 human ovarian carcinoma cells. Measuredby its binding ability in a functional ELISA. Immobilized rhMIS at 3 μg/Ml (100μL/well) will bind rrMIS RII/Fc Chimera with a linear range of 1.6-100 ng/mL. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| Purity : |
>97%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstituteat 10μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin. |
| Storage : |
Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution. |
