Active Recombinant Human Anti-Mullerian Hormone

• Cat.No.: AMH-383H
• Product Overview: RecombinantHuman AMH protein was expressed in E. coli. Ala453-Arg560.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 453-560 a.a.
• Description: AMH is atissue-specific TGF-β superfamily growth factor. Its expression is restrictedto the Sertoli cells of fetal and postnatal testis, and to the granulosacells of postnatal ovary. The human MIS gene encodes a 553 amino acid residue(aa) prepropeptide containing a signal a sequence (1-24), a proregion (25-455),and the carboxylterminal bioactive protein (44-6553). MIS is synthesized andsecreted as a disulfide-linked homodimeric pro-protein. Proteolytic cleavageis required to generate the N-terminal pro-region and the C-terminal bioactiveprotein, which remain associated in a noncovalent complex.
• Form: Lyophilizedfrom a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
• N-terminalSequence: Ala453
• MolecularWeight: 11.7 kDa (monomer)
• Activity: Measuredby its ability to inhibit growth of OVCAR-3 human ovarian carcinoma cells. Measuredby its binding ability in a functional ELISA. Immobilized rhMIS at 3 μg/Ml (100μL/well) will bind rrMIS RII/Fc Chimera with a linear range of 1.6-100 ng/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 10μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: AMH anti-Mullerian hormone [ Homosapiens ]
• Official Symbol: AMH
• Synonyms: AMH; anti-Mullerianhormone; MIF;MIS; muellerian-inhibiting factor; Mullerian inhibiting factor; Mullerianinhibiting substance; muellerian-inhibiting substance
• Gene ID: 268
• mRNA Refseq: NM_000479
• Protein Refseq: NP_000470
• MIM: 600957
• UniProt ID: P03971
• Chromosome Location: 19p13.3
• Pathway: Cytokine-cytokine receptor interaction; ALK2signaling events; TGF-beta signaling pathway
• Function: growth factor activity; hormone activity; receptorbinding; transforming growth factor beta receptor binding