"Gpc2" Related Products


Recombinant Mouse Glypican 2 (cerebroglycan), His-tagged

Cat. No.: Gpc2-5625M
Product Overview: RecombinantMouse Gpc2 protein was expressed in Murine myeloma cell line. Lys27-Arg358& Ala359-Leu554, with a C-terminal 6-His tag.
Description: Theglypicans (GPC) constitute a family of heparan sulfate proteoglycans that areattached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor.Six members of this family have been identified in mammals (GPC1-GPC6). Allglypican core proteins contain an N-terminal signal peptide, a large globularcysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-likeregion containing the heparan sulfate attachment sites, and a C-terminal GPIattachment site. Based on the degree of their amino acid sequence similarity,two subfamilies of glypicans have been defined.
Source: Murine myeloma cellline, NS0-derived.
Form: Lyophilized from a0.2 um filtered solution in Tris-Citrate and NaCl.
N-terminalSequence: Lys27 & Ala359
MolecularWeight: 58.9 kDa
Activity: Measuredby its binding ability in a functional ELISA. Immobilized rm Gpc2 at 4μg/mL(100μL/well) can bind rhMidkine with a linear range of 4-200 ng/mL.
Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE: 60 kDa, non-reducingconditions.
Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstituteat 100μg/mL in PBS.
Storage: Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.
Gene Name: Gpc2 glypican 2 (cerebroglycan)[ Mus musculus ]
Official Symbol: Gpc2
Synonyms: Gpc2; glypican 2(cerebroglycan); 2410016G05Rik; glypican-2
Gene ID: 71951
mRNA Refseq: NM_172412
Protein Refseq: NP_766000
UniProt ID: Q3TU56
Chromosome Location: 5
Function: heparan sulfateproteoglycan binding

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