Recombinant Human G Protein-Coupled Receptor 114, His-tagged GPR114-5630H

Recombinant Human G Protein-Coupled Receptor 114, His-tagged

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Recombinant Human G Protein-Coupled Receptor 114, His-tagged

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Cat. No. : GPR114-5630H
Product Overview : RecombinantHuman GPR114 protein was expressed in Chinese HamsterOvary cell line. Glu22-Gly184, with a C-terminal 6-His tag.
Description : GPR114 isa member of the LN-TM7 family of adhesion-type 7-transmembrane (TM) G-proteincoupled receptors (GPCR) that exhibit long extracellular N-termini. The 528amino acid (aa) human GPR114 sequence predicts a 21 aa signal sequence, a 229aa N-terminal extracellular domain (ECD) and seven TM regions separated by shortintracellular and extracellular regions. Like other LN-TM7 members, the ECDcontains a highly glycosylated mucin-like stalk that is predicted to functionin adhesion, followed by a GPCR proteolytic cleavage site (GPS) it does not containany other easily recognizable domains.
Source : Chinese HamsterOvary cell line, CHO-derived.
Form : Lyophilized from a0.2 um filtered solution in PBS.
N-terminalSequence : Glu22
MolecularWeight : 19.4 kDa
Activity : Measuredby the ability of the immobilized protein to enhance the adhesion of thehuman T98G glioblastoma cells (ATCC: CRL-1690) to human Fibronectin. When 4x104cells/well are added to plates coated with human Fibronectin (0.1 μg/mL) andrhGPR114 (10 μg/well, 100 μL/well) for 60 minutes at 37°C, there is a 2-4fold increase in adhesion to human fibronectin induced by the presence ofrhGPR114.
Endotoxin Level : <1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE : 40-55kDa, reducing conditions.
Purity : >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : Reconstituteat 100μg/mL in PBS.
Storage : Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.
Gene Name : GPR114 G protein-coupledreceptor 114 [ Homo sapiens ]
Official Symbol : GPR114
Synonyms : GPR114; Gprotein-coupled receptor 114; PGR27; probable G-proteincoupled receptor 114; G protein-coupled receptor PGR27; G-protein coupledreceptor PGR27
Gene ID : 221188
mRNA Refseq : NM_153837
Protein Refseq : NP_722579
UniProt ID : Q8IZF4
Chromosome Location : 16q13
Function : G-protein coupledreceptor activity; receptor activity; signal transducer activity

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