Recombinant Mouse Cysteine-Rich Secretory Protein 4, His-tagged


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Recombinant Mouse Cysteine-Rich Secretory Protein 4, His-tagged

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Cat. No. : Crisp4-5612M
Product Overview : Recombinant Mouse Crisp4protein was expressed in CHO with a C-terminal 6-His tag. Pro22-Lys250.
Description : Crisp4 is one ofseveral CRISPs (cysteine-rich secretory proteins) that contain 16 invariantcysteine residues and show structural homolgy to snake, insect, and lizardvenom proteins. These proteins consist of an N-terminal SCP/CAP domain, ahinge region, and a cysteine-rich CRISP domain. CRISPs are expressed indiscrete but overlapping regions of the male reproductive tract and play arole in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation,and the acrosomal reaction.
Source : Chinese HamsterOvary cell line, CHO-derived
Form : Lyophilized from a0.2 μm filtered solution in PBS.
N-terminalSequence : Leu23
Molecular Weight : 27.2 kDa
Activity : Measuredby the ability of the immobilized protein to support the adhesion of NTera-2human testicular embryonic carcinoma cells. The ED50 for thiseffect is typically 0.3-1.5 μg/mL after 1 hour incubation at 37 °C. Optimaldilutions should be determined by each laboratory for each application.
Endotoxin Level : <1.0 EUper 1 μg of the protein by the LAL method.
SDS-PAGE : 34-37kDa, reducing conditions
Purity : >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : Reconstituteat 200 μg/mL in PBS
Storage : Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
Gene Name : Crisp4 cysteine-rich secretoryprotein 4 [ Mus musculus ]
Official Symbol : Crisp4
Synonyms : Crisp4; cysteine-richsecretory protein 4; 9230112K08Rik; cysteine-rich secretory protein 1;Uncharacterized protein
Gene ID : 78081
mRNA Refseq : NM_030033
Protein Refseq : NP_084309
UniProt ID : Q9D259
Chromosome Location : 1 A3; 1
Function : molecular function
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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