"Dhh" Related Products

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Recombinant Mouse Desert Hedgehog

Cat. No.: Dhh-393M
Product Overview: Recombinantmouse Dhh protein was expressed in E. coli. Cys23-Gly198, with anN-terminal Met.
Description: Dhh belongs to thehighly conserved Hedgehog family of proteins which are involved in multipledevelopmental processes. Hedgehogs are synthesized as 45 kDa precursors thatare cleaved autocatalytically.
Source: E. coli
Form: Lyophilized from a0.2 μm filtered solution in PBS and DTT.
N-terminal Sequence: Met
Molecular Weight: 20.1 kDa
Activity: Measured by itsability to induce alkaline phosphatase production by MC3T3E1 mouseosteoblastic cells. The ED50 for this effect is typically 5-20 μg/mL.
Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstitute at 500μg/mL in sterile PBS.
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.
Gene Name: Dhhdesert hedgehog [ Mus musculus ]
Official Symbol: Dhh
Synonyms: Dhh; deserthedgehog; C78960;deserthedgehog protein; HHG-3
Gene ID: 13363
mRNA Refseq: NM_007857
Protein Refseq: NP_031883
UniProt ID: Q61488
Chromosome Location: 15
Pathway: Hedgehog SignalingPathway
Function: Calcium ion binding; hydrolase activity; patchedbinding; peptidase activity; protein binding; zinc ion binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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