| Cat. No. : |
Dkk2-401M |
| Description : |
Dkk2 is a member ofthe Dickkopf family of secreted Wnt modulators. Dkk proteins contain a signalpeptide and two conserved cysteine-rich domains that are separated by alinker region. The second cysteine-rich domain mediates Dkk2 bindingactivities, and its interaction with LRP beta propellers has been mapped. |
| Source : |
Spodopterafrugiperda, Sf 21 (baculovirus)-derived |
| Form : |
Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| N-terminal Sequence : |
Ser26 |
| Molecular Weight : |
27.1 kDa |
| Activity : |
Measured by itsability to inhibit Wnt-3a-induced alkaline phosphates production in thepresence of BMP2 by MC3T3-E1 mouse osteoblastic cells. Measured in acompetitive binding assay. When Recombinant Mouse Kremen1 is immobilized at 4μg/mL (100 μL/well), Recombinant Mouse Dkk2 inhibits 50% binding ofbiotinylated Recombinant Human Dkk1 (200 ng/mL) at the concentration range of2-8 μg/mL. |
| Endotoxin Level : |
<1.0 EU per 1 μgof the protein by the LAL method. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstitute at 100μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin. |
| Storage : |
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution. |
