Active Recombinant Mouse Serine Protease Inhibitor, Kunitz Type 1, His-tagged

• Cat.No.: Spint1-405M
• Product Overview: Recombinantmouse Spint1 protein was expressed in Murine myeloma cellline, NS0-derived, with a C-terminal 10-His tag. Glu30-Glu443.

Specification

• Cat. No.: Spint1-405M
• Description: The protein encodedby this gene is a member of the Kunitz family of serine protease inhibitors.The protein is a potent inhibitor specific for HGF activator and is thoughtto be involved in the regulation of the proteolytic activation of HGF ininjured tissues. Alternative splicing results in multiple variants encodingdifferent isoforms.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in Tris.
• N-terminal Sequence: Glu30
• Molecular Weight: 48 kDa
• Activity: Measured by itsability to inhibit trypsin cleavage of a fluorogenic peptide substrate,Mca-RPKPVE-Nval-WRK(Dnp)-NH2, The IC50 value is <1.5 nM, as measured under the described conditions. See Activity AssayProtocol.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 100μg/mL in sterile, deionized water.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70°C as supplied. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Spint1serine protease inhibitor, Kunitz type 1 [ Mus musculus ]
• Official Symbol: Spint1
• Synonyms: Spint1;serine protease inhibitor, Kunitz type 1; kunitz-type protease inhibitor 1;HAI 1; HAI; HAI-1; HAI1; Hepatocyte growth factor activator inhibitor type 1;Kunitz type protease inhibitor 1; Kunitz-type protease inhibitor 1; MANSC2;Serine peptidase inhibitor Kunitz type 1; SPINT1; SPIT1_HUMAN;OTTMUSP00000016930; OTTMUSP00000016931; OTTMUSP00000016932; hepatocyte growthfactor activator inhibitor type 1
• Gene ID: 20732
• mRNA Refseq: NM_016907
• Protein Refseq: NP_058603
• UniProt ID: Q9R097
• Chromosome Location: 2
• Pathway: Transcriptionalmisregulation in cancers
• Function: peptidaseinhibitor activity; serine-type endopeptidase inhibitor activity