"Spint1" Related Products

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Recombinant Mouse Serine Protease Inhibitor, Kunitz Type 1, His-tagged

Cat. No.: Spint1-405M
Product Overview: Recombinantmouse Spint1 protein was expressed in Murine myeloma cellline, NS0-derived, with a C-terminal 10-His tag. Glu30-Glu443.
Description: The protein encodedby this gene is a member of the Kunitz family of serine protease inhibitors.The protein is a potent inhibitor specific for HGF activator and is thoughtto be involved in the regulation of the proteolytic activation of HGF ininjured tissues. Alternative splicing results in multiple variants encodingdifferent isoforms.
Source: Murine myeloma cellline, NS0-derived
Form: Lyophilized from a0.2 μm filtered solution in Tris.
N-terminal Sequence: Glu30
Molecular Weight: 48 kDa
Activity: Measured by itsability to inhibit trypsin cleavage of a fluorogenic peptide substrate,Mca-RPKPVE-Nval-WRK(Dnp)-NH2, The IC50 value is <1.5 nM, as measured under the described conditions. See Activity AssayProtocol.
Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: Reconstitute at 100μg/mL in sterile, deionized water.
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70°C as supplied. 3months, -20 to -70°C under sterile conditions after reconstitution.
Gene Name: Spint1serine protease inhibitor, Kunitz type 1 [ Mus musculus ]
Official Symbol: Spint1
Synonyms: Spint1;serine protease inhibitor, Kunitz type 1; kunitz-type protease inhibitor 1;HAI 1; HAI; HAI-1; HAI1; Hepatocyte growth factor activator inhibitor type 1;Kunitz type protease inhibitor 1; Kunitz-type protease inhibitor 1; MANSC2;Serine peptidase inhibitor Kunitz type 1; SPINT1; SPIT1_HUMAN;OTTMUSP00000016930; OTTMUSP00000016931; OTTMUSP00000016932; hepatocyte growthfactor activator inhibitor type 1
Gene ID: 20732
mRNA Refseq: NM_016907
Protein Refseq: NP_058603
UniProt ID: Q9R097
Chromosome Location: 2
Pathway: Transcriptionalmisregulation in cancers
Function: peptidaseinhibitor activity; serine-type endopeptidase inhibitor activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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